Journalartikel

Jahr der Veröffentlichung: 2015

Seiten: 215-221

Zeitschrift: International Immunopharmacology

Bandnummer: 29

Heftnummer: 1

ISSN: 1567-5769

eISSN: 1878-1705

DOI Link: https://doi.org/10.1016/j.intimp.2015.09.013

Verlag: Elsevier

Abstract: 

Recent studies showed that the non-neuronal cholinergic system (NNCS) is taking part in bone metabolism. Most studies investigated its role in osteoblasts, but up to now, the involvement of the NNCS in human osteoclastogenesis remains relatively unclear. Thus, aim of the present study was to determine whether the application of acetylcholine (ACh, 10(-4) M), nicotine (10(-6) M), mineralized collagen membranes or brain derived neurotrophic factor (BDNF, 40 ng/mL) influences the mRNA regulation of molecular components of the NNCS and the neurotrophin family during osteoclastogenesis.

Peripheral blood mononuclear cells (PBMCs) were isolated from the blood of young healthy donors (n = 8) and incubated with bone fragments and osteoclast differentiation media for 21 days. All the results are based on the measurement of RNA.

Real-time RT-PCR analysis demonstrated a down-regulation of nicotinic acetylcholine receptor (nAChR) subunit alpha 2 and muscarinic acetylcholine receptor (mAChR) M3 by osteoclastogenesis while BDNF mRNA expression was not regulated. Application of ACh, nicotine, BDNF or collagen membranes did not affect osteoclastic differentiation. No regulation was detected for nAChR subunit alpha 7, tropomyosin-related kinase receptor B (TrkB), and choline acetyltransferase (ChAT).

Taken together, we assume that the transcriptional level of osteoclastogenesis of healthy young humans is not regulated by BDNF, ACh, and nicotine. Thus, these drugs do not seem to worsen bone degradation and might therefore be suitable as modulators of bone substitution materials if having a positive effect on bone formation. (C) 2015 Elsevier B.V. All rights reserved.

Harvard-Zitierstil: Ternes, S., Trinkaus, K., Bergen, I., Knaack, S., Gelinsky, M., Kilian, O., et al. (2015) Impact of acetylcholine and nicotine on human osteoclastogenesis in vitro, International Immunopharmacology, 29(1), pp. 215-221. https://doi.org/10.1016/j.intimp.2015.09.013

APA-Zitierstil: Ternes, S., Trinkaus, K., Bergen, I., Knaack, S., Gelinsky, M., Kilian, O., Heiss, C., & Lips, K. (2015). Impact of acetylcholine and nicotine on human osteoclastogenesis in vitro. International Immunopharmacology. 29(1), 215-221. https://doi.org/10.1016/j.intimp.2015.09.013