Journalartikel

Jahr der Veröffentlichung: 2015

Seiten: 119-126

Zeitschrift: International Immunopharmacology

Bandnummer: 29

Heftnummer: 1

ISSN: 1567-5769

eISSN: 1878-1705

DOI Link: https://doi.org/10.1016/j.intimp.2015.07.021

Verlag: Elsevier

Abstract: 

Acetylcholine (ACh) is an important signaling molecule in non-neuronal systems where it is involved in regulation of viability, proliferation, differentiation and migration of mesenchymal stem cells (MSC) that are capable to differentiate into osteoblasts, chondrocytes and adipocytes. Patients with the systemic disease osteoporosis show altered MSC properties, reduced bone formation and mineral density followed by increased bone fragility and high fracture incidence. Here we asked whether nicotinic and muscarinic acetylcholine receptors (AChR) are expressed in osteoblasts, adipocytes and chondrocytes differentiated from bone MSC extracted from human reaming debris (RDMSC) that was harvested during surgery of long bone diaphyseal fractures.

Using RT-PCR, AChR were detected in RDMSC, osteoblasts, chondrocytes and adipocytes of male and female bone-healthy and of female osteoporotic donors. An up-regulation in multiplicity and occurrence of AChR subtypes was found in female compared to male donors and in osteoblast of male donors compared to adipocytes. Real-time RT-PCR analysis resulted in a significant increase in relative expression of nAChR alpha 9 in chondrocytes compared to adipocytes of healthy female donors. The nAChR subunit alpha 10 was significantly up-regulated in osteoblasts of healthy compared to osteoporotic donors as well as the mAChR M5 that is additionally decreased in osteoporotic osteoblasts compared to MSC and chondrocytes of osteoporotic donors.

In summary, the gene expression of AChR during differentiation of RDMSC and its regulation in cells of osteoporotic donors lead to the assumption that AChR signaling is involved in bone formation and might be utilized to stimulate bone remodeling processes. (C) 2015 Elsevier B.V. All rights reserved.

Harvard-Zitierstil: Zablotni, A., Dakischew, O., Trinkaus, K., Hartmann, S., Szalay, G., Heiss, C., et al. (2015) Regulation of acetylcholine receptors during differentiation of bone mesenchymal stem cells harvested from human reaming debris, International Immunopharmacology, 29(1), pp. 119-126. https://doi.org/10.1016/j.intimp.2015.07.021

APA-Zitierstil: Zablotni, A., Dakischew, O., Trinkaus, K., Hartmann, S., Szalay, G., Heiss, C., & Lips, K. (2015). Regulation of acetylcholine receptors during differentiation of bone mesenchymal stem cells harvested from human reaming debris. International Immunopharmacology. 29(1), 119-126. https://doi.org/10.1016/j.intimp.2015.07.021