Journal article

Publication year: 2015

Pages: 208-214

Journal: International Immunopharmacology

Volume number: 29

Issue number: 1

ISSN: 1567-5769

eISSN: 1878-1705

DOI Link: https://doi.org/10.1016/j.intimp.2015.06.029

Publisher: Elsevier

Abstract: 

Butyrylcholinesterase (BChE) degrades acetylcholine in addition to acetylcholinesterase (AChE) which is involved in embryonic development of limbs. Since BChE is expressed by osteoblast-like cells we asked whether it is functional in adult bone remodeling. We addressed this issue by analyzing BChE gene-deficient mice (BChE-KO).

Bones were extracted from 16-week old female BChE-KO and corresponding wild type mice (WT). Femoral bones were used for biomechanical testing and mu CT evaluation of cancellous and cortical bone. Also vertebrae Th12 and L1 were investigated with mu CT while L3 was used for tartrate-resistant acidic phosphatase (TRAP) histomorphometry and Th10 for gene expression analysis by means of real-time RT-PCR.

BChE-KO did not reveal significant differences in biomechanical bone strength and bone mineral density determined by mu CT. Microarchitecture of cancellous and cortical bone showed an increase in mu CT parameters like trabecular thickness, trabecular separation, and relative cortical bone area of femoral BChE-KO bone compared to WT. In vertebrae no changes of microstructure and mRNA expression were detected. However, osteoclast histomorphometry with TRAP stained sections demonstrated a significant increase in relative osteoclast number. In conclusion, in adult murine bone the role of BChE is limited to bone specific changes in microarchitecture and to an increase in relative number of bone resorbing osteoclasts whereas the main collagen resorbing enzyme Cathepsin-K (CtsK) was stably expressed. Besides, AChE might be able to compensate the lack of BChE. Thus, further analyses using bone tissue specific AChE BChE cre-lox double knockout mice would be helpful. (C) 2015 Elsevier B.V. All rights reserved.

Harvard Citation style: Haupt, M., Kauschke, V., Sender, J., Kampschulte, M., Kovtun, A., Duerselen, L., et al. (2015) Bone status of adult female butyrylcholinesterase gene-deficient mice, International Immunopharmacology, 29(1), pp. 208-214. https://doi.org/10.1016/j.intimp.2015.06.029

APA Citation style: Haupt, M., Kauschke, V., Sender, J., Kampschulte, M., Kovtun, A., Duerselen, L., Heiss, C., & Lips, K. (2015). Bone status of adult female butyrylcholinesterase gene-deficient mice. International Immunopharmacology. 29(1), 208-214. https://doi.org/10.1016/j.intimp.2015.06.029