Journal article
Authors list: Fatykhova, Diana; Rabes, Anne; Machnik, Christoph; Guruprasad, Kunchur; Pache, Florence; Berg, Johanna; Toennies, Mario; Bauer, Torsten T.; Schneider, Paul; Schimek, Maria; Eggeling, Stephan; Mitchell, Timothy J.; Mitchell, Andrea M.; Hilker, Rolf; Hain, Torsten; Suttorp, Norbert; Hippenstiel, Stefan; Hocke, Andreas C.; Opitz, Bastian
Publication year: 2015
Journal: PLoS ONE
Volume number: 10
Issue number: 8
ISSN: 1932-6203
Open access status: Gold
DOI Link: https://doi.org/10.1371/journal.pone.0137108
Publisher: Public Library of Science
Abstract:
Streptococcus pneumoniae is a major cause of pneumonia, sepsis and meningitis. The pore-forming toxin pneumolysin is a key virulence factor of S. pneumoniae, which can be sensed by the NLRP3 inflammasome. Among the over 90 serotypes, serotype 1 pneumococci (particularly MLST306) have emerged across the globe as a major cause of invasive disease. The cause for its particularity is, however, incompletely understood. We therefore examined pneumococcal infection in human cells and a human lung organ culture system mimicking infection of the lower respiratory tract. We demonstrate that different pneumococcal serotypes differentially activate inflammasome-dependent IL-1 beta production in human lung tissue and cells. Whereas serotype 2, 3, 6B, 9N pneumococci expressing fully haemolytic pneumolysins activate NLRP3 inflammasome-dependent responses, serotype 1 and 8 strains expressing non-haemolytic toxins are poor activators of IL-1 beta production. Accordingly, purified haemolytic pneumolysin but not serotype 1-associated non-haemolytic toxin activates strong IL-1 beta production in human lungs. Our data suggest that the evasion of inflammasome-dependent innate immune responses by serotype 1 pneumococci might contribute to their ability to cause invasive diseases in humans.
Citation Styles
Harvard Citation style: Fatykhova, D., Rabes, A., Machnik, C., Guruprasad, K., Pache, F., Berg, J., et al. (2015) Serotype 1 and 8 Pneumococci Evade Sensing by Inflammasomes in Human Lung Tissue, PLoS ONE, 10(8), Article e0137108. https://doi.org/10.1371/journal.pone.0137108
APA Citation style: Fatykhova, D., Rabes, A., Machnik, C., Guruprasad, K., Pache, F., Berg, J., Toennies, M., Bauer, T., Schneider, P., Schimek, M., Eggeling, S., Mitchell, T., Mitchell, A., Hilker, R., Hain, T., Suttorp, N., Hippenstiel, S., Hocke, A., & Opitz, B. (2015). Serotype 1 and 8 Pneumococci Evade Sensing by Inflammasomes in Human Lung Tissue. PLoS ONE. 10(8), Article e0137108. https://doi.org/10.1371/journal.pone.0137108
Keywords
HOST-DEFENSE; NLRP3 INFLAMMASOME; PNEUMOLYSIN; PNEUMONIAE VIRULENCE FACTORS
