Journalartikel

Jahr der Veröffentlichung: 2015

Seiten: 398-404

Zeitschrift: Experimental and Clinical Endocrinology & Diabetes

Bandnummer: 123

Heftnummer: 7

ISSN: 0947-7349

eISSN: 1439-3646

DOI Link: https://doi.org/10.1055/s-0035-1549936

Verlag: Thieme Publishing

Abstract: 
In this study we investigated changes of serum leptin in 74 newborn lambs and associations with environmental temperature (from -8 degrees C to +25 degrees C), body temperature, and concentrations of plasma lipids, 3-beta-hydroxybutyric acid and blood glucose. A leptin radioimmunoassay was established, using an antiserum (rabbit) produced against a partial sequence of ovine leptin (31-44). Before measurement, serum samples were denatured. The sensitivity of the assay was 0.4 mu gl(-1) and intra- and interassay coefficients of variation were 5.1% and 2.5%, respectively. Blood samples were collected immediately after birth up to 24h postnatally (pn). Median leptin concentrations at birth and 24h pn were 20.9 and 52.7 mu gl(-1), respectively. Because of non-normal distribution, leptin concentrations were converted to log(leptin) before further statistical processing. The change in log(leptin) during the first 24h was highly significant (p<0.0001). Correlation analysis showed significant associations between serum leptin and the following variables: environmental temperature 24h pn (r=0.34, p<0.005), log(plasma triglycerides) 24h pn (r=0.50, p<0.001), log(plasma 3-beta-hydroxybutyric acid) 24h pn (r=-0.50, p<0.001), blood glucose 6h pn (r=0.43, p<0.001) and plasma cholesterol 12h pn (r=0.38, p=0.001). We conclude that this radioimmunoassay is suited to measure total serum ovine leptin and that total leptin is already regulated in the very early postnatal phase. Leptin is increased at higher environmental temperatures, consistent with leptin's suppressive effect on energy expenditure and appetite. Furthermore, leptin levels are associated with plasma concentrations of lipids and lipid metabolites.

Harvard-Zitierstil: Schilling, J., Hospes, R., Kaya, G., Failing, K., Gortner, L., Wudy, S., et al. (2015) Serum Leptin in Neonatal Lambs is Associated with Temperature, Plasma Lipids and Metabolites, Experimental and Clinical Endocrinology & Diabetes, 123(7), pp. 398-404. https://doi.org/10.1055/s-0035-1549936

APA-Zitierstil: Schilling, J., Hospes, R., Kaya, G., Failing, K., Gortner, L., Wudy, S., & Blum, W. (2015). Serum Leptin in Neonatal Lambs is Associated with Temperature, Plasma Lipids and Metabolites. Experimental and Clinical Endocrinology & Diabetes. 123(7), 398-404. https://doi.org/10.1055/s-0035-1549936