Journal article

Publication year: 2014

Pages: 384-392

Journal: Cells Tissues Organs

Volume number: 199

Issue number: 5-6

ISSN: 1422-6405

eISSN: 1422-6421

DOI Link: https://doi.org/10.1159/000371341

Publisher: Karger Publishers

Abstract: 
Bacterial colonization of implant surfaces is a feared complication in surgery and orthopedics. Due to the increasing number of periprosthetic infections caused by multidrugresistant microorganisms, new antibacterial coatings for biomaterials must be developed. The excellent antibacterial properties of silver nanoparticles (AgNPs) against multidrugresistant bacteria, for example, have been repeatedly described. For this reason, we tested a nanosilver-doped polymethylmethacrylate (PMMA) bone cement and a nanosilver-coated titanium alloy regarding their influence on osteoclastogenesis of primary human peripheral blood mononuclear cells. Both implant variants did not inhibit osteoclast differentiation. Excellent cell attachment and unaltered podosomal structures were confirmed. Additionally, no induction of oxidative or endoplasmic reticulum stress could be observed. However, PMMA loaded with gentamicin and nanosilver inhibited preosteoclast fusion and further osteoclastogenesis. The material also led to decreased clathrin-dependent endocytosis as well as decreased levels of endoplasmic reticulum stress. Therefore, biomaterial functionalization with AgNPs did not disturb osteoclastogenesis, while addition of gentamicin reduced the cytocompatibility of nanosilver-doped materials towards human osteoclasts. (C) 2015 S. Karger AG, Basel

Harvard Citation style: Pauksch, L., Franke, J., Schnettler, R. and Lips, K. (2014) Synergistic Toxicity of Gentamicin- and Nanosilver-Doped Polymethylmethacrylate Bone Cement on Primary Human Osteoclasts, Cells Tissues Organs, 199(5-6), pp. 384-392. https://doi.org/10.1159/000371341

APA Citation style: Pauksch, L., Franke, J., Schnettler, R., & Lips, K. (2014). Synergistic Toxicity of Gentamicin- and Nanosilver-Doped Polymethylmethacrylate Bone Cement on Primary Human Osteoclasts. Cells Tissues Organs. 199(5-6), 384-392. https://doi.org/10.1159/000371341