Journal article

Publication year: 2015

Pages: 160-168

Journal: Chemical Engineering Science

Volume number: 126

ISSN: 0009-2509

eISSN: 1873-4405

DOI Link: https://doi.org/10.1016/j.ces.2014.12.020

Publisher: Elsevier

Abstract: 
Micro bubble aeration in stirred bioreactors represents a suitable method that allows sufficient oxygen transfer without the necessity of intense stirring. However, understanding of micro bubble behavior in real culture media remains a crucial factor in the interpretation of possible cell/bubble interactions and the scale up of bioprocesses. The present study focuses the impact of various stirrer configurations, sparging rates, as well as 2 different bioreactor scales on micro-bubbles in SF-900 If insect culture medium, which represents a suitable example for Plutonic F68 supplemented culture media commonly applied in cell culture processes. Obtained results for the first time demonstrate a coalescence determined increase in bubble Sauter diameter d(32) from 187 to 211 mu m with increasing stirring intensities. The development of an empirical model reveals d(32) infinity V-0.4(P/V)(0.4), were P represents the stirrer induced power input and V the medium volume. The results were interpreted a consequence of micro-bubble entrapment in turbulent flow structures. Additional studies on the volumetric oxygen transfer coefficient k(L)a demonstrate a strong impact of axial flow impellers and indicate that increases in k(L)a can be attributed to an improvement of the gas-liquid oxygen transfer rather than an increased specific gas surface area clue to bubble breakup. (C) 2014 Elsevier Ltd. All rights reserved.

Harvard Citation style: Druzinec, D., Salzig, D., Kraume, M. and Czermak, P. (2015) Micro-bubble aeration in turbulent stirred bioreactors: Coalescence behavior in Pluronic F68 containing cell culture media, Chemical Engineering Science, 126, pp. 160-168. https://doi.org/10.1016/j.ces.2014.12.020

APA Citation style: Druzinec, D., Salzig, D., Kraume, M., & Czermak, P. (2015). Micro-bubble aeration in turbulent stirred bioreactors: Coalescence behavior in Pluronic F68 containing cell culture media. Chemical Engineering Science. 126, 160-168. https://doi.org/10.1016/j.ces.2014.12.020