Journal article

Publication year: 2015

Pages: 306-316

Journal: The Journal of Infectious Diseases

Volume number: 211

Issue number: 2

ISSN: 0022-1899

eISSN: 1537-6613

Open access status: Green

DOI Link: https://doi.org/10.1093/infdis/jiu428

Publisher: Oxford University Press

Abstract: 
Microbial infections can induce aberrant responses in cellular stress pathways, leading to translational attenuation, metabolic restriction, and activation of oxidative stress, with detrimental effects on cell survival. Here we show that infection of human airway epithelial cells with Streptococcus pneumoniae leads to induction of endoplasmic reticulum (ER) and oxidative stress, activation of mitogen-associated protein kinase (MAPK) signaling pathways, and regulation of their respective target genes. We identify pneumococcal H2O2 as the causative agent for these responses, as both catalase-treated and pyruvate oxidase-deficient bacteria lacked these activities. Pneumococcal H2O2 induced nuclear NF-kappa B translocation and transcription of proinflammatory cytokines. Inhibition of translational arrest and ER stress by salubrinal or of MAPK signaling pathways attenuate cytokine transcription. These results provide strong evidence for the notion that inhibition of translation is an important host pathway in monitoring harmful pathogen-associated activities, thereby enabling differentiation between pathogenic and nonpathogenic bacteria.

Harvard Citation style: Loose, M., Hudel, M., Zimmer, K., Garcia, E., Hammerschmidt, S., Lucas, R., et al. (2015) Pneumococcal Hydrogen Peroxide-Induced Stress Signaling Regulates Inflammatory Genes, The Journal of Infectious Diseases, 211(2), pp. 306-316. https://doi.org/10.1093/infdis/jiu428

APA Citation style: Loose, M., Hudel, M., Zimmer, K., Garcia, E., Hammerschmidt, S., Lucas, R., Chakraborty, T., & Pillich, H. (2015). Pneumococcal Hydrogen Peroxide-Induced Stress Signaling Regulates Inflammatory Genes. The Journal of Infectious Diseases. 211(2), 306-316. https://doi.org/10.1093/infdis/jiu428