Journal article
Authors list: Pilatz, A.; Ceylan, I.; Schuppe, H. C.; Ludwig, M.; Fijak, M.; Chakraborty, T.; Weidner, W.; Bergmann, M.; Wagenlehner, F.
Publication year: 2015
Pages: 160-167
Journal: Andrologia
Volume number: 47
Issue number: 2
ISSN: 0303-4569
eISSN: 1439-0272
DOI Link: https://doi.org/10.1111/and.12239
Publisher: Wiley
Abstract:
The objective of this study was to investigate spermatogenesis and testicular inflammation in a rat model of unilateral Escherichia coli epididymitis in a long-term follow-up. Unilateral epididymitis was induced in 30 Sprague-Dawley rats by injecting E.coli into the right ductus deferens. Oral antimicrobial treatment with sparfloxacin (50mgkg(-1) body weight/7days) was administered in half of the animals 24h after infection. Five treated and five untreated rats were killed at 2weeks, 3months and 6months after infection. Spermatogenesis was investigated using a histological semi-quantitative score. The presence of inflammatory cells (B- and T lymphocytes, macrophages and granulocytes) in the testicular tissues was evaluated by immunohistochemistry. The testes were sterile at all times. Over the course of 6months, spermatogenesis underwent significant incremental impairment on the inoculated side as compared to the contralateral side (P<0.001). However, overall spermatogenesis scores were not significantly different between treated and untreated animals (P>0.3 at each time point). Finally, loss of testicular architecture on the inoculated side was not associated with any cellular inflammatory response. Thus, adjuvant therapies need to be studied, and research is necessary on how to prevent deterioration of testicular function in bacterial epididymitis.
Citation Styles
Harvard Citation style: Pilatz, A., Ceylan, I., Schuppe, H., Ludwig, M., Fijak, M., Chakraborty, T., et al. (2015) Experimental Escherichia coli epididymitis in rats: assessment of testicular involvement in a long-term follow-up, Andrologia, 47(2), pp. 160-167. https://doi.org/10.1111/and.12239
APA Citation style: Pilatz, A., Ceylan, I., Schuppe, H., Ludwig, M., Fijak, M., Chakraborty, T., Weidner, W., Bergmann, M., & Wagenlehner, F. (2015). Experimental Escherichia coli epididymitis in rats: assessment of testicular involvement in a long-term follow-up. Andrologia. 47(2), 160-167. https://doi.org/10.1111/and.12239
Keywords
ACUTE EPIDIDYMITIS; CHLAMYDIA-TRACHOMATIS; EPIDIDIMOORCHITIS; OFLOXACIN; ORCHITIS; RAT MODEL; testicular inflammation
