Journal article
Authors list: Arenz, Andrea; Ziemann, Frank; Mayer, Christina; Wittig, Andrea; Dreffke, Kirstin; Preising, Stefanie; Wagner, Steffen; Klussmann, Jens-Peter; Engenhart-Cabillic, Rita; Wittekindt, Claus
Publication year: 2014
Pages: 839-846
Journal: Journal of Radiation Oncology, Biology, Physics
Volume number: 190
Issue number: 9
ISSN: 0179-7158
eISSN: 1439-099X
DOI Link: https://doi.org/10.1007/s00066-014-0605-5
Publisher: Springer
Background and purpose. Human Papillomavirus (HPV)-related head and neck squamous cell carcinoma (HNSCC) respond favourably to radiotherapy as compared to HPV-unrelated HNSCC. We investigated DNA damage response in HPV-positive and HPV-negative HNSCC cell lines aiming to identify mechanisms, which illustrate reasons for the increased sensitivity of HPV-positive cancers of the oropharynx. Methods. Radiation response including clonogenic survival, apoptosis, DNA double-strand break (DSB) repair, and cell cycle redistribution in four HPV-positive (UM-SCC-47, UM-SCC-104, 93-VU-147T, UPCI:SCC152) and four HPV-negative (UD-SCC-1, UM-SCC-6, UM-SCC-11b, UT-SCC-33) cell lines was evaluated. Results. HPV-positive cells were more radiosensitive (mean SF2: 0.198 range: 0.22-0.18) than HPV-negative cells (mean SF2: 0.34, range: 0.45-0.27; p = 0.010). Irradiated HPV-positive cell lines progressed faster through S-phase showing a more distinct accumulation in G2/M. The abnormal cell cycle checkpoint activation was accompanied by a more pronounced increase of cell death after x-irradiation and a higher number of residual and unreleased DSBs. Conclusions. The enhanced responsiveness of HPV-related HNSCC to radiotherapy might be caused by a higher cellular radiosensitivity due to cell cycle dysregulation and impaired DNA DSB repair.
Abstract:
Citation Styles
Harvard Citation style: Arenz, A., Ziemann, F., Mayer, C., Wittig, A., Dreffke, K., Preising, S., et al. (2014) Increased radiosensitivity of HPV-positive head and neck cancer cell lines due to cell cycle dysregulation and induction of apoptosis, Journal of Radiation Oncology, Biology, Physics, 190(9), pp. 839-846. https://doi.org/10.1007/s00066-014-0605-5
APA Citation style: Arenz, A., Ziemann, F., Mayer, C., Wittig, A., Dreffke, K., Preising, S., Wagner, S., Klussmann, J., Engenhart-Cabillic, R., & Wittekindt, C. (2014). Increased radiosensitivity of HPV-positive head and neck cancer cell lines due to cell cycle dysregulation and induction of apoptosis. Journal of Radiation Oncology, Biology, Physics. 190(9), 839-846. https://doi.org/10.1007/s00066-014-0605-5
Keywords
CARCINOMA; CISPLATIN; DNA damage repair; head and neck cancer; HPV-related oropharyngeal cancer; HUMAN-PAPILLOMAVIRUS TYPE-16; Radiosensitivity
