Journal article
Authors list: Scheil-Bertram, Stefanie; Kappler, Roland; von Baer, Alexandra; Hartwig, Erich; Sarkar, Michael; Serra, Massimo; Bruederlein, Silke; Westhoff, Bettina; Melzner, Ingo; Bassaly, Birgit; Herms, Jochen; Hugo, Heinz-Hermann; Schulte, Michael; Moeller, Peter
Publication year: 2014
Pages: 1041-1055
Journal: International Journal of Oncology
Volume number: 44
Issue number: 4
ISSN: 1019-6439
eISSN: 1791-2423
Open access status: Hybrid
DOI Link: https://doi.org/10.3892/ijo.2014.2268
Publisher: Spandidos Publications
Abstract:
The molecular basis of chordoma is still poorly understood, particularly with respect to differentially expressed genes involved in the primary origin of chordoma. In this study, therefore, we compared the transcriptional expression profile of one sacral chordoma recurrence, two chordoma cell lines (U-CH1 and U-CH2) and one chondrosarcoma cell line (U-CS2) with vertebral disc using a high-density oligonucleotide array. The expression of 65 genes whose mRNA levels differed significantly (p<0.001; 6-fold change) between chordoma and control (vertebral disc) was identified. Genes with increased expression in chordoma compared to control and chondrosarcoma were most frequently located on chromosomes 2 (11%), 5 (8%), 1 and 7 (each 6%), whereas interphase cytogenetics of 33 chordomas demonstrated gains of chromosomal material most prevalent on 7q (42%), 12q (21%), 17q (21%), 20q (27%) and 22q (21%). The microarray data were confirmed for selected genes by quantitative polymerase chain reaction analysis. As in other studies, we showed the expression of brachyury. We demonstrate the expression of new potential candidates for chordoma tumorigenesis, such as CD24, ECRG4, RARRES2, IGFBP2, RAP1, HAI2, RAB38, osteopontin, GalNAc-T3, VAMP8 and others. Thus, we identified and validated a set of interesting candidate genes whose differential expression likely plays a role in chordoma.
Citation Styles
Harvard Citation style: Scheil-Bertram, S., Kappler, R., von Baer, A., Hartwig, E., Sarkar, M., Serra, M., et al. (2014) Molecular profiling of chordoma, International Journal of Oncology, 44(4), pp. 1041-1055. https://doi.org/10.3892/ijo.2014.2268
APA Citation style: Scheil-Bertram, S., Kappler, R., von Baer, A., Hartwig, E., Sarkar, M., Serra, M., Bruederlein, S., Westhoff, B., Melzner, I., Bassaly, B., Herms, J., Hugo, H., Schulte, M., & Moeller, P. (2014). Molecular profiling of chordoma. International Journal of Oncology. 44(4), 1041-1055. https://doi.org/10.3892/ijo.2014.2268
Keywords
BRACHYURY; chondrosarcoma; chordoma; comparative genomic hybridization; GeneChip; LOW-GRADE
