Journal article

TGF-β directs trafficking of the epithelial sodium channel ENaC which has implications for ion and fluid transport in acute lung injury


Authors listPeters, Dorothea M.; Vadasz, Istvan; Wujak, Lukasz; Wygrecka, Malgorzata; Olschewski, Andrea; Becker, Christin; Herold, Susanne; Papp, Rita; Mayer, Konstantin; Rummel, Sebastian; Brandes, Ralph P.; Guenther, Andreas; Waldeggerg, Siegfried; Eickelberg, Oliver; Seeger, Werner; Morty, Rory E.

Publication year2014

PagesE374-E383

JournalProceedings of the National Academy of Sciences

Volume number111

Issue number3

ISSN0027-8424

Open access statusGreen

DOI Linkhttps://doi.org/10.1073/pnas.1306798111

PublisherNational Academy of Sciences


Abstract
TGF-beta is a pathogenic factor in patients with acute respiratory distress syndrome (ARDS), a condition characterized by alveolar edema. A unique TGF-beta pathway is described, which rapidly promoted internalization of the alpha beta gamma epithelial sodium channel (ENaC) complex from the alveolar epithelial cell surface, leading to persistence of pulmonary edema. TGF-beta applied to the alveolar airspaces of live rabbits or isolated rabbit lungs blocked sodium transport and caused fluid retention, which-together with patch-clamp and flow cytometry studies-identified ENaC as the target of TGF-beta. TGF-beta rapidly and sequentially activated phospholipase D1, phosphatidylinositol-4- phosphate 5-kinase 1 alpha, and NADPH oxidase 4 (NOX4) to produce reactive oxygen species, driving internalization of beta ENaC, the subunit responsible for cell-surface stability of the alpha beta gamma ENaC complex. ENaC internalization was dependent on oxidation of beta ENaC Cys(43). Treatment of alveolar epithelial cells with bronchoalveolar lavage fluids from ARDS patients drove beta ENaC internalization, which was inhibited by a TGF-beta neutralizing antibody and a Tgfbr1 inhibitor. Pharmacological inhibition of TGF-beta signaling in vivo in mice, and genetic ablation of the nox4 gene in mice, protected against perturbed lung fluid balance in a bleomycin model of lung injury, highlighting a role for both proximal and distal components of this unique ENaC regulatory pathway in lung fluid balance. These data describe a unique TGF-beta-dependent mechanism that regulates ion and fluid transport in the lung, which is not only relevant to the pathological mechanisms of ARDS, but might also represent a physiological means of acutely regulating ENaC activity in the lung and other organs.



Citation Styles

Harvard Citation stylePeters, D., Vadasz, I., Wujak, L., Wygrecka, M., Olschewski, A., Becker, C., et al. (2014) TGF-β directs trafficking of the epithelial sodium channel ENaC which has implications for ion and fluid transport in acute lung injury, Proceedings of the National Academy of Sciences, 111(3), pp. E374-E383. https://doi.org/10.1073/pnas.1306798111

APA Citation stylePeters, D., Vadasz, I., Wujak, L., Wygrecka, M., Olschewski, A., Becker, C., Herold, S., Papp, R., Mayer, K., Rummel, S., Brandes, R., Guenther, A., Waldeggerg, S., Eickelberg, O., Seeger, W., & Morty, R. (2014). TGF-β directs trafficking of the epithelial sodium channel ENaC which has implications for ion and fluid transport in acute lung injury. Proceedings of the National Academy of Sciences. 111(3), E374-E383. https://doi.org/10.1073/pnas.1306798111



Keywords


alveolar epitheliumBIOPHYSICAL PROPERTIESCELL-SURFACE EXPRESSIONCLEARANCECONDUCTANCEfluid homeostasisNA+ CHANNELNADPH OXIDASESUBIQUITIN LIGASE NEDD4L

Last updated on 2025-10-06 at 10:16