Journal article

Publication year: 2013

Pages: 667-675

Journal: Journal of Surgical Research

Volume number: 185

Issue number: 2

ISSN: 0022-4804

eISSN: 1095-8673

DOI Link: https://doi.org/10.1016/j.jss.2013.07.061

Publisher: Elsevier

Abstract: 

Background: A scaffold for treatment of deep osteochondral defects should be stable, integrate well, and provide a surface for chondrocytes. To meet these demands, a biphasic scaffold of allogenous sterilized bone with a collagen surface was developed. Integration was tested in the sheep model.

Material and methods: Cartilage chips were taken from the nonweight-bearing area of the left knee of 12 sheep and cultured. After 4 wk a second procedure followed and defects of 9.4-mm diameter at the weight-bearing area of the medial femoral condyle of the right knee were created. The sterilized scaffold was inserted and the cultured autologous chondrocytes were dripped onto the surface. After 6 wk, 3 mo, and 6 mo the animals were sacrificed; the explanted femoral condyles were evaluated macroscopically and using histologic, immunohistochemical, and electronmicroscopic methods.

Results: After 6 wk the level of the surface was well preserved, after 3 mo parts of the scaffold were sintered but after 6 mo the surface was continuous. Full integration of the allogenous bone could be observed after 6 mo. The surface of the scaffold after 6 wk consisted of bone, but after 3 mo some chondrocytes and after 6 mo a continuous chondral layer could be detected.

Conclusions: The biphasic scaffold of allogenous bone and collagen proved to be stable and sufficiently integrated in the short-and midterm interval. Whether the chondrocytes on the surface had been derived from implanted chondrocytes or the scaffold with its surface was sufficiently chondroconductive must be answered in further investigations. (C) 2013 Elsevier Inc. All rights reserved.

Harvard Citation style: Schleicher, I., Lips, K., Sommer, U., Schappat, I., Martin, A., Szalay, G., et al. (2013) Allogenous bone with collagen for repair of deep osteochondral defects, Journal of Surgical Research, 185(2), pp. 667-675. https://doi.org/10.1016/j.jss.2013.07.061

APA Citation style: Schleicher, I., Lips, K., Sommer, U., Schappat, I., Martin, A., Szalay, G., & Schnettler, R. (2013). Allogenous bone with collagen for repair of deep osteochondral defects. Journal of Surgical Research. 185(2), 667-675. https://doi.org/10.1016/j.jss.2013.07.061