Journalartikel

Jahr der Veröffentlichung: 2013

Seiten: 1212-1215

Zeitschrift: Acta Crystallographica Section F: Structural Biology Communications

Bandnummer: 69

eISSN: 2053-230X

Open Access Status: Green

DOI Link: https://doi.org/10.1107/S1744309113025761

Verlag: International Union of Crystallography

Abstract: 
The secreted pore-forming toxin listeriolysin O (LLO) from the intracellular pathogen Listeria monocytogenes is a member of the family of cholesterol-dependent cytolysins (CDC) with broad properties in pathogenesis. Its role as a virulence factor is enigmatic: it disrupts membranes and acts as an inductor of both pro- and anti-inflammatory responses in infected cells. In addition, LLO is also a potent target for immunogenicity during infection. Natively secreted LLO from a recombinant L. innocua strain was crystallized in its water-soluble monomeric form. The crystals obtained belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 26.7, b = 85.1, c = 230.0 angstrom, and diffracted to beyond 2.2 angstrom resolution. The Matthews coefficient and the solvent content were estimated to be 2.4 angstrom(3) Da(-1) and 49.2%, respectively. The structure with one molecule in the asymmetric unit was solved using Phaser employing the structure of the previously characterized CDC toxin perfringolysin O as a search model.

Harvard-Zitierstil: Koester, S., Hudel, M., Chakraborty, T. and Yildiz, O. (2013) Crystallization and X-ray crystallographic analysis of the cholesterol-dependent cytolysin listeriolysin O from Listeria monocytogenes, Acta Crystallographica Section F: Structural Biology Communications, 69, pp. 1212-1215. https://doi.org/10.1107/S1744309113025761

APA-Zitierstil: Koester, S., Hudel, M., Chakraborty, T., & Yildiz, O. (2013). Crystallization and X-ray crystallographic analysis of the cholesterol-dependent cytolysin listeriolysin O from Listeria monocytogenes. Acta Crystallographica Section F: Structural Biology Communications. 69, 1212-1215. https://doi.org/10.1107/S1744309113025761