Journal article

Publication year: 2013

Pages: 133-141

Journal: Peptides

Volume number: 47

ISSN: 0196-9781

DOI Link: https://doi.org/10.1016/j.peptides.2013.07.013

Publisher: Elsevier

Abstract: 
Little is known about interactions between immune and neuro-endocrine systems in patients with septic shock. We therefore evaluated whether the corticotropin-releasing hormone (CRH) and/or proopiomelanocortin (POMC) derivatives [ACTH, p-endorphin (p-END), p-lipotropin (beta-LPH), alpha-melanocyte stimulating hormone (alpha-MSH) or N-acetyl-beta-END (Nac-beta-END)] have any influences on monocyte deactivation as a major factor of immunosuppression under septic shock conditions. Sixteen patients with septic shock were enrolled in a double-blind, cross-over and placebo controlled clinical study; 0.5 mu g/(kg(bodyweight) h) CRH (or placebo) were intravenously administered for 24 h. Using flow cytometry we investigated the immunosuppression in patients as far as related to the loss of leukocyte surface antigen-DR expression on circulating monocytes (mHLA-DR). ACTH, beta-END immunoreacive material (IRM), beta-LPH IRM, alpha-MSH and Nac-beta-END IRM as well as TNF-alpha and mHLA-DR expression were determined before, during and after treatment with CRH (or placebo). A significant correlation between plasma concentration of ce.-MSH and mHLA-DR expression and an inverse correlation between mHLA-DR expression and TNF-alpha plasma level were found. Additionally, a significant increase of mHLA-DR expression was observed 16 h after starting the CRH infusion; 8 h later, the mHLA-DR expression had decreased again. Our results indicate that the up-regulation of mHLA-DR expression after CRH infusion is not dependent on the release of POMC derivatives. From the correlation between plasma concentration of alpha-MSH and mHLA-DR expression, we conclude that in patients with septic shock the down-regulation of mHAL-DR expression is accompanied by the loss of monocytic release of ce.-MSH into the cardiovascular compartment. (C) 2013 Elsevier Inc. All rights reserved.

Harvard Citation style: Matejec, R., Kayser, F., Schmal, F., Uhle, F., Boedeker, R., Maxeiner, H., et al. (2013) Effects of corticotropin-releasing hormone on proopiomelanocortin derivatives and monocytic HLA-DR expression in patients with septic shock, Peptides, 47, pp. 133-141. https://doi.org/10.1016/j.peptides.2013.07.013

APA Citation style: Matejec, R., Kayser, F., Schmal, F., Uhle, F., Boedeker, R., Maxeiner, H., & Kolbe, J. (2013). Effects of corticotropin-releasing hormone on proopiomelanocortin derivatives and monocytic HLA-DR expression in patients with septic shock. Peptides. 47, 133-141. https://doi.org/10.1016/j.peptides.2013.07.013