Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles - Forschungsportal der Justus-Liebig-Universität Gießen:

Journalartikel

Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles

Autorenliste: Konrad, L.; Kuhnert, S.; Nayudu, P. L.; Einspanier, R.; Hinsch, K. D.; Hinsch, E.

Jahr der Veröffentlichung: 2012

Seiten: 349-353

Zeitschrift: Andrologia

Bandnummer: 44

ISSN: 0303-4569

DOI Link: https://doi.org/10.1111/j.1439-0272.2011.01188.x

Verlag: Wiley

Abstract:
In mammals, the oocyte and preimplantation embryo are protected by the zona pellucida (ZP) consisting mainly of ZP glycoproteins, which are responsible for sperm binding, induction of the acrosome reaction and zona pellucida hardening to prevent polyspermia. The ZP proteins become increasingly important as possible predictors for in vitro cultured oocytes competence. As little is known about the stage-dependent expression of ZP1, ZP2 and ZP3 in marmoset monkey (Callithrix jacchus) oocytes, mRNA expression was investigated with real-time RT-PCR. Total-RNA was isolated from three different classes of marmoset oocytes; Class 1 oocytes from periantral follicles (<600 mu m, n = 10), Class 2 oocytes from small antral follicles (600-1000 mu m, n = 10) and Class 3 oocytes from large antral follicles (>1000 mu m, n = 9). Compared with Class 1 oocytes mRNA expression of ZP1, ZP2 and ZP3 in Class 2 oocytes was significantly decreased. In Class 3 oocytes, the transcription of ZP1, ZP2 and ZP3 genes showed also a significant decrease compared with Class 1 oocytes. In this study a differently regulated expression of the ZP genes during late folliculogenesis with an obvious downregulation of ZP1, ZP2 and ZP3 could be demonstrated for the first time in the marmoset monkey.

Zitierstile

Harvard-Zitierstil: Konrad, L., Kuhnert, S., Nayudu, P., Einspanier, R., Hinsch, K. and Hinsch, E. (2012) Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles, Andrologia, 44, pp. 349-353. https://doi.org/10.1111/j.1439-0272.2011.01188.x

APA-Zitierstil: Konrad, L., Kuhnert, S., Nayudu, P., Einspanier, R., Hinsch, K., & Hinsch, E. (2012). Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles. Andrologia. 44, 349-353. https://doi.org/10.1111/j.1439-0272.2011.01188.x

Zitierstile

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