Journal article

Publication year: 2012

Pages: 545-553

Journal: Microbes and Infection

Volume number: 14

Issue number: 6

ISSN: 1286-4579

eISSN: 1769-714X

Open access status: Bronze

DOI Link: https://doi.org/10.1016/j.micinf.2012.01.004

Publisher: Elsevier

Abstract: 

Mycobacterium aVi14177 subspecies paratuberculosis (MAP) is suspected to be a causative agent in Crohn's disease. Recent evidence suggests that MAP can induce the expression of Matrix Metalloproteinases (MMPs), which are the main proteases in the pathogenesis of mucosal ulcerations in inflammatory bowel disease (IBD). Within the present study, we analysed whether oral MAP exposure can induce colonic MMP expression in vivo.

In MAP exposed mice MAP and spheroplasts were visualized in intramucosal leukocyte aggregates. MAP exposed mice exhibited a higher colonic expression of Mmp-2, -9, -13, -14, Timp-1, Tlr2, Tlr6, Il-1 beta, and Tnf-alpha. Cell clusters of MIVIP-9 positive cells adjacent to intramucosal leukocyte aggregates and CD45(+) leukocytes were identified as the major cellular sources of MMP-9. Enhanced TLR2 expression was visualized on the luminal side of colonic enterocytes.

Although MAP exposure did not lead to macroscopic intestinal inflammation, the observed MAP spheroplasts in intramucosal leukocyte aggregates together with increased colonic expression of toll-like receptors, pro-inflammatory eytokines, and MMPs upon MAP exposure represents a part of the host immune response towards MAP. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Harvard Citation style: Roderfeld, M., Koc, A., Rath, T., Bloecher, S., Tschuschner, A., Alcineden, O., et al. (2012) Induction of matrix metalloproteinases and TLR2 and 6 in murine colon after oral exposure to Mycobacterium avium subsp paratuberculosis, Microbes and Infection, 14(6), pp. 545-553. https://doi.org/10.1016/j.micinf.2012.01.004

APA Citation style: Roderfeld, M., Koc, A., Rath, T., Bloecher, S., Tschuschner, A., Alcineden, O., Fischer, M., von Gerlach, S., Goethe, R., Eckelt, E., Meens, J., Buelte, M., Basler, T., & Roeb, E. (2012). Induction of matrix metalloproteinases and TLR2 and 6 in murine colon after oral exposure to Mycobacterium avium subsp paratuberculosis. Microbes and Infection. 14(6), 545-553. https://doi.org/10.1016/j.micinf.2012.01.004