Journal article

Publication year: 2012

Pages: 485-492

Journal: Cellular Physiology and Biochemistry

Volume number: 29

Issue number: 3-4

ISSN: 1015-8987

eISSN: 1421-9778

Open access status: Gold

DOI Link: https://doi.org/10.1159/000338502

Publisher: Karger Publishers Open Access

Abstract: 
Aims: The study was aimed to investigate whether nicotine affects endothelial expression of PTHrP and PTHrP receptor, a peptide system involved in endothelial protection against apoptosis. Methods: Isolated and cultured rat coronary endothelial cells were used. Immunoblot techniques were used to study activation of mitogen-activated protein (MAP) kinases and to quantify PTHrP and PTHrP receptor expression. Real-time RT-PCR was used to quantify PTHrP, PTHrP-receptor, bcl-2, and bax mRNA expression. The rate of apoptosis was determined by HOE33258 staining and confirmed by quantification of the bcl-2-to-bax ratio. In vitro data were compared to hearts from rats exposed to cigarette smoking. Results: Nicotine induced PTHrP protein expression at nanomolar levels and small increases of PTHrP release (approximate to 8%). Antagonists directed against the alpha 7 subunit of cholinergic receptors, the most prominent isoform, attenuated nicotine-dependent increases of PTHrP expression. This effect of nicotine was p38 MAPK dependent. Nicotine at micromolar concentrations reduced PTHrP receptor expression. In vitro and in vivo we found a correlation between PTHrP receptor expression and bcl-2 expression. Conclusion: Nicotine induces PTHrP expression in endothelial cells but excessive concentrations of nicotine reduce PTHrP receptor expression thereby attenuating any protective effects of PTHrP against apoptosis. Copyright (C) 2012 S. Karger AG, Basel

Harvard Citation style: Roethig, A., Schreckenberg, R., Weber, K., Conzelmann, C., Rebelo, R. and Schlueter, K. (2012) Effects of Nicotine on PTHrP and PTHrP Receptor Expression in Rat Coronary Endothelial Cells, Cellular Physiology and Biochemistry, 29(3-4), pp. 485-492. https://doi.org/10.1159/000338502

APA Citation style: Roethig, A., Schreckenberg, R., Weber, K., Conzelmann, C., Rebelo, R., & Schlueter, K. (2012). Effects of Nicotine on PTHrP and PTHrP Receptor Expression in Rat Coronary Endothelial Cells. Cellular Physiology and Biochemistry. 29(3-4), 485-492. https://doi.org/10.1159/000338502