Journal article

Publication year: 2012

Pages: 100-106

Journal: European Journal of Pharmacology

Volume number: 681

Issue number: 1-3

ISSN: 0014-2999

DOI Link: https://doi.org/10.1016/j.ejphar.2012.02.001

Publisher: Elsevier

Abstract: 

The aim of the present study was to find out the mechanism by which the inflammatory mediator, bradykinin, induces an increase of the cytosolic Ca2+ concentration ([Ca2+] i) in enteric neurons. For this purpose, ganglia in the isolated submucosa from rat colon were loaded with the Ca2+-sensitive dye, fura-2, and were exposed to bradykinin (2 . 10(-8) mol/l). Under control conditions, the kinin evoked a transient increase in [Ca2+](i). Preincubation with quinacrine or arachidonyltrifluoromethylketone (AACOCF3), i. e. blockers of cytosolic phospholipase A2, prevented the raise of [Ca2+](i). This inhibition was mimicked by 5,8,11,14-eicosatetrayonic acid (ETYA), an inhibitor of cyclooxygenases as well as lipoxygenases, and by BWA4C, a selective inhibitor of lipoxygenases, whereas indomethacin was ineffective, suggesting the mediation of the kinin response by a lipoxygenase metabolite. Indeed, a leukotriene, leukotriene D4 (LTD4), mimicked the effect of bradykinin. The LTD4 receptor blocker, MK-571, inhibited the increase in [Ca2+](i) evoked by LTD4 and by bradykinin. Consequently, bradykinin receptors in submucosal ganglia from rat colon are coupled to a stimulation of phospholipase A2, the release of arachidonic acid and the production of LTD4, which seems to be finally responsible for the change in the cytosolic Ca2+ concentration.

Harvard Citation style: Rehn, M. and Diener, M. (2012) Cysteinyl leukotrienes mediate the response of submucosal ganglia from rat colon to bradykinin, European Journal of Pharmacology, 681(1-3), pp. 100-106. https://doi.org/10.1016/j.ejphar.2012.02.001

APA Citation style: Rehn, M., & Diener, M. (2012). Cysteinyl leukotrienes mediate the response of submucosal ganglia from rat colon to bradykinin. European Journal of Pharmacology. 681(1-3), 100-106. https://doi.org/10.1016/j.ejphar.2012.02.001