Journal article

Publication year: 2011

Pages: 1366-1373

Journal: Bioconjugate Chemistry

Volume number: 22

Issue number: 7

ISSN: 1043-1802

DOI Link: https://doi.org/10.1021/bc200063m

Publisher: American Chemical Society

Abstract: 
A novel method for regulating the activity of homodimeric proteins "molecular gate" approach was proposed and its usefulness illustrated for the type II restriction endonuclease SsoII (R.Ssoll) as a model. The "molecular gate" approach is based on the modification of R.Ssoll with azobenzene derivatives, which allows regulating DNA binding and cleavage via illumination with light. R.SsoII variants with single cysteine residues introduced at selected positions were obtained and modified with maleimidoazobenzene derivatives. A twofold change in the enzymatic activity after illumination with light of wavelengths of 365 and 470 nm, respectively, was demonstrated when one or two molecules of azobenzene derivatives were attached to the R.Ssoll at the entrance of or within the DNA-binding site.

Harvard Citation style: Le Thi Hien, Zatsepin, T., Schierling, B., Volkov, E., Wende, W., Pingoud, A., et al. (2011) Restriction Endonuclease Ssoll with Photoregulated Activity-a "Molecular Gate" Approach, Bioconjugate Chemistry, 22(7), pp. 1366-1373. https://doi.org/10.1021/bc200063m

APA Citation style: Le Thi Hien, Zatsepin, T., Schierling, B., Volkov, E., Wende, W., Pingoud, A., Kubareva, E., & Oretskaya, T. (2011). Restriction Endonuclease Ssoll with Photoregulated Activity-a "Molecular Gate" Approach. Bioconjugate Chemistry. 22(7), 1366-1373. https://doi.org/10.1021/bc200063m