Journalartikel

Jahr der Veröffentlichung: 2010

Seiten: 267-276

Zeitschrift: Cardiovascular Research

Bandnummer: 88

Heftnummer: 2

ISSN: 0008-6363

DOI Link: https://doi.org/10.1093/cvr/cvq273

Verlag: Oxford University Press

Abstract: 

We tested whether long-term caloric restriction (CR) corrects pre-existing manifestations of cardiac ageing in rats.

The age-specific effects of CR (-40%, 6 months) on mortality, left ventricular (LV) function, mitochondrial function, oxidative damage, and apoptosis were analysed in young (6 + 6 months) and senescent rats (24 + 6 months). CR in senescent rats significantly reduced mortality. LV and cardiomyocyte hypertrophy were reduced together with the mRNA expression and plasma concentrations of overload indicators BNP/ANP. Mitochondrial function was improved, resulting in lower oxidative damage and apoptotic activation. In particular, the pro-apoptotic Bcl-xS/Bcl-xL isoform pattern, mitochondrial translocation of Bax, release of cytochrome C into cytosol, and caspase-9 activation were reduced in comparison to age-matched rats on the control diet. However, CR resulted only in minor changes in young rats. Serum obtained from old control or CR rats was used for in vitro experiments. H9C2 cardiomyoblasts and adult rat ventricular cardiomyocytes preconditioned with CR serum demonstrated a low Bcl-xS/Bcl-xL ratio. H9C2 cells were resistant against H(2)O(2)-mediated loss of mitochondrial membrane potential, apoptosis activation, and reduced cell viability. Thus, beneficial effects of CR are mediated through circulating factors and can be mimicked with CR serum. However, this protection critically depended on a high Bcl-xL protein expression as seen after siRNA-mediated Bcl-xL knockdown.

CR is cardioprotective in senescent myocardium by correcting pre-existing mitochondrial dysfunction and apoptotic activation and by preventing deterioration in LV function. Therefore, interventions that mimic these effects of CR may represent an additional therapeutic option for the aged or failing heart.

Harvard-Zitierstil: Niemann, B., Chen, Y., Issa, H., Silber, R. and Rohrbach, S. (2010) Caloric restriction delays cardiac ageing in rats: role of mitochondria, Cardiovascular Research, 88(2), pp. 267-276. https://doi.org/10.1093/cvr/cvq273

APA-Zitierstil: Niemann, B., Chen, Y., Issa, H., Silber, R., & Rohrbach, S. (2010). Caloric restriction delays cardiac ageing in rats: role of mitochondria. Cardiovascular Research. 88(2), 267-276. https://doi.org/10.1093/cvr/cvq273