Journalartikel

Jahr der Veröffentlichung: 2010

Seiten: 1890-1U43

Zeitschrift: Arteriosclerosis, Thrombosis, and Vascular Biology

Bandnummer: 30

Heftnummer: 10

ISSN: 1079-5642

eISSN: 1524-4636

Open Access Status: Bronze

DOI Link: https://doi.org/10.1161/ATVBAHA.110.209692

Verlag: American Heart Association

Abstract: 
Bone marrow-derived progenitor cells have been implicated to contribute to neointima formation, but the time course and extent of their accumulation and differentiation into vascular cells and, most importantly, the long-term contribution of bone marrow-derived progenitor cells to the vascular lesion remain undefined. Methods and Results-Wire-induced injury of the femoral artery was performed on chimeric C57BL/6 mice transplanted with bone marrow from transgenic mice expressing enhanced green fluorescence protein, and vessels were harvested at 3 days, 1, 2, 3, 4, 6, and 16 weeks after dilatation (n = 8 animals per time point). Using high-resolution microscopy, we unexpectedly found that the expression of smooth muscle cell or endothelial cell markers in enhanced green fluorescence protein positive cells was a very rare event. Indeed, most of the enhanced green fluorescence protein positive cells that accumulated during the acute inflammatory response were identified as monocytes/macrophages, and their number declined at later time points. In contrast, a substantial fraction of highly proliferative stem cell antigen-1 and CD34(+) but enhanced green fluorescence protein negative and thus locally derived cells were detected in the adventitia. Conclusion-These data provide evidence that the differentiation of bone marrow-derived progenitor cells into smooth muscle cell or endothelial cell lineages seems to be an exceedingly rare event. Moreover, the contribution of bone marrow-derived cells to the cellular compartment of the neointima is limited to a transient period of the inflammatory response. (Arterioscler Thromb Vasc Biol. 2010;30:1890-1896.)

Harvard-Zitierstil: Daniel, J., Bielenberg, W., Stieger, P., Weinert, S., Tillmanns, H. and Sedding, D. (2010) Time-Course Analysis on the Differentiation of Bone Marrow-Derived Progenitor Cells Into Smooth Muscle Cells During Neointima Formation, Arteriosclerosis, Thrombosis, and Vascular Biology, 30(10), pp. 1890-1U43. https://doi.org/10.1161/ATVBAHA.110.209692

APA-Zitierstil: Daniel, J., Bielenberg, W., Stieger, P., Weinert, S., Tillmanns, H., & Sedding, D. (2010). Time-Course Analysis on the Differentiation of Bone Marrow-Derived Progenitor Cells Into Smooth Muscle Cells During Neointima Formation. Arteriosclerosis, Thrombosis, and Vascular Biology. 30(10), 1890-1U43. https://doi.org/10.1161/ATVBAHA.110.209692