Journalartikel
Autorenliste: Maxeiner, Hagen; Krehbiehl, Nina; Mueller, Andrea; Woitasky, Nadine; Akintuerk, Hakan; Mueller, Matthias; Weigand, Markus A.; Abdallah, Yaser; Kasseckert, Sascha; Schreckenberg, Rolf; Schlueter, Klaus-Dieter; Wenzel, Sibylle
Jahr der Veröffentlichung: 2010
Seiten: 730-737
Zeitschrift: European Journal of Heart Failure
Bandnummer: 12
Heftnummer: 7
ISSN: 1388-9842
eISSN: 1879-0844
Open Access Status: Bronze
DOI Link: https://doi.org/10.1093/eurjhf/hfq063
Verlag: Wiley
Cardiac progenitor cells (CPCs) have been shown to promote cardiac regeneration in vivo. Understanding the function of CPCs is essential for further implementation of these cells in the treatment of cardiac diseases. The present study tested the hypothesis that adult CPC exert paracrine effects that lead to an improvement in the functional characteristics of cardiomyocytes. This study also investigated whether aging (we included patients aged between 4 months and 81 years) has any effect on the paracrine mechanisms of CPC. The supernatant of CPC generated both from human and rat hearts-so called 'conditioned cardiosphere medium' improved the contractile behaviour of isolated adult cardiomyocytes in a concentration-dependent manner after incubation for 24 h and increased the SERCA/NCX ratio. The observed positive effects on contractile behaviour were independent of the CPC donors' age. Conditioned cardiosphere media also normalized angiotensin II-induced contractile dysfunction. Cytokines released by CPC into the media were detected by cytokine arrays. The observed diversity of cytokines released by CPC needs to be further elucidated in detail. Nevertheless, CPC are a promising therapeutic approach in the field of cardiac disease. The methods described allow investigation of the underlying paracrine mechanisms in a standardized in vitro situation.
Abstract:
Zitierstile
Harvard-Zitierstil: Maxeiner, H., Krehbiehl, N., Mueller, A., Woitasky, N., Akintuerk, H., Mueller, M., et al. (2010) New insights into paracrine mechanisms of human cardiac progenitor cells, European Journal of Heart Failure, 12(7), pp. 730-737. https://doi.org/10.1093/eurjhf/hfq063
APA-Zitierstil: Maxeiner, H., Krehbiehl, N., Mueller, A., Woitasky, N., Akintuerk, H., Mueller, M., Weigand, M., Abdallah, Y., Kasseckert, S., Schreckenberg, R., Schlueter, K., & Wenzel, S. (2010). New insights into paracrine mechanisms of human cardiac progenitor cells. European Journal of Heart Failure. 12(7), 730-737. https://doi.org/10.1093/eurjhf/hfq063
Schlagwörter
Cardiac progenitor cells; cardioprotection; Conditioned media; CONTRACTILE FUNCTION; HEART-FAILURE; MESENCHYMAL STEM-CELLS; MULTIPOTENT
