Journalartikel
Autorenliste: Xiong, Chenling; Yang, Guang; Kumar, Sanjiv; Aggarwal, Saurabh; Leustik, Martin; Snead, Connie; Hamacher, Juerg; Fischer, Bernhard; Umapathy, Nagavedi S.; Hossain, Hamid; Wendel, Albrecht; Catravas, John D.; Verin, Alexander D.; Fulton, David; Black, Stephen M.; Chakraborty, Trinad; Lucas, Rudolf
Jahr der Veröffentlichung: 2010
Seiten: 207-213
Zeitschrift: Vascular Pharmacology
Bandnummer: 52
Heftnummer: 5-6
ISSN: 1537-1891
Open Access Status: Green
DOI Link: https://doi.org/10.1016/j.vph.2009.12.010
Verlag: Elsevier
Abstract:
Listeriosis can lead to potentially lethal pulmonary complications in newborns and immune compromised patients, characterized by extensive permeability edema. Listeriolysin (LLO), the main virulence factor of Listeria monocytogenes, induces a dose-dependent hyperpermeability in monolayers of human lung microvascular endothelial cells in vitro. The permeability increasing activity of LLO, which is accompanied by an increased reactive oxygen species (ROS) generation, RhoA activation and myosin light chain (MLC) phosphorylation, can be completely inhibited by the protein kinase C (PKC) alpha/beta inhibitor GO6976, indicating a crucial role for PKC in the induction of barrier dysfunction. The TNF-derived TIP peptide, which mimics the lectin-like domain of the cytokine, blunts LLO-induced hyperpermeability in vitro, upon inhibiting LLD-induced protein kinase C-alpha activation, ROS generation and MLC phosphorylation and upon restoring the RhoA/Rac 1 balance. These results indicate that the lectin-like domain of TNF has a potential therapeutic value in protecting from LLO-induced pulmonary endothelial hyperpermeability. (C) 2010 Elsevier Inc. All rights reserved.
Zitierstile
Harvard-Zitierstil: Xiong, C., Yang, G., Kumar, S., Aggarwal, S., Leustik, M., Snead, C., et al. (2010) The lectin-like domain of TNF protects from listeriolysin-induced hyperpermeability in human pulmonary microvascular endothelial cells - A crucial role for protein kinase C-α inhibition, Vascular Pharmacology, 52(5-6), pp. 207-213. https://doi.org/10.1016/j.vph.2009.12.010
APA-Zitierstil: Xiong, C., Yang, G., Kumar, S., Aggarwal, S., Leustik, M., Snead, C., Hamacher, J., Fischer, B., Umapathy, N., Hossain, H., Wendel, A., Catravas, J., Verin, A., Fulton, D., Black, S., Chakraborty, T., & Lucas, R. (2010). The lectin-like domain of TNF protects from listeriolysin-induced hyperpermeability in human pulmonary microvascular endothelial cells - A crucial role for protein kinase C-α inhibition. Vascular Pharmacology. 52(5-6), 207-213. https://doi.org/10.1016/j.vph.2009.12.010
Schlagwörter
BARRIER DYSFUNCTION; EDEMA REABSORPTION; Endothelial hyperpermeability; Listeriolysin; LUNG TRANSPLANTATION; MONOCYTOGENES; PERMEABILITY; Protein kinase C; TNF
