Journal article

Publication year: 2010

Pages: 655-666

Journal: European Respiratory Journal

Volume number: 35

Issue number: 3

ISSN: 0903-1936

eISSN: 1399-3003

Open access status: Bronze

DOI Link: https://doi.org/10.1183/09031936.00059709

Publisher: European Respiratory Society

Abstract: 

Although increasing numbers of patients suffer from chronic destructive lung diseases, there are no effective therapeutic options apart from transplantation. Understanding the mechanisms of physiological and regenerative alveolar septation is prerequisite for the development of regenerative therapies for the lung. We compared lung gene expression in the phase of induction of post-natal and post-pneumonectomy alveolarisation to identify regulatory genes involved in both processes.

We performed genome-wide microarray screenings of newborn and pneumonectomised mouse lungs 1 and 3 days after birth or surgery. Selected candidates were validated by real-time PCR, Western blot and in situ hybridisation.

We found 58 genes to be regulated in both models with 40 candidates being changed likewise. Many of these genes participated in growth and differentiation processes. Additionally, immune system, structural molecules, respiratory chain, signal transduction and metabolism were involved. Some candidates were not yet linked to specific functions. The highest regulatory concordance was observed for various isoforms of (pro-)collagen molecules, elastin and the elastin-associated protein fibrillin1 being corporately upregulated.

Our findings do not definitively support a common regulating mechanism for induction of postnatal and adult alveolarisation, but some candidates in the intersection of both models are promising for further investigations.

Harvard Citation style: Wolff, J., Wilhelm, J., Fink, L., Seeger, W. and Voswinckel, R. (2010) Comparative gene expression profiling of post-natal and post-pneumonectomy lung growth, European Respiratory Journal, 35(3), pp. 655-666. https://doi.org/10.1183/09031936.00059709

APA Citation style: Wolff, J., Wilhelm, J., Fink, L., Seeger, W., & Voswinckel, R. (2010). Comparative gene expression profiling of post-natal and post-pneumonectomy lung growth. European Respiratory Journal. 35(3), 655-666. https://doi.org/10.1183/09031936.00059709