Journalartikel
Autorenliste: Richter, Jan F.; Gitter, Alfred H.; Guenzel, Dorothee; Weiss, Siegfried; Mohamed, Walid; Chakraborty, Trinad; Fromm, Michael; Schulzke, Joerg D.
Jahr der Veröffentlichung: 2009
Seiten: G1350-G1359
Zeitschrift: American Journal of Physiology - Gastrointestinal and Liver Physiology
Bandnummer: 296
Heftnummer: 6
ISSN: 0193-1857
eISSN: 1522-1547
DOI Link: https://doi.org/10.1152/ajpgi.00040.2009
Verlag: American Physiological Society
Abstract:
Richter JF, Gitter AH, Gunzel D, Weiss S, Mohamed W, Chakraborty T, Fromm M, Schulzke JD. Listeriolysin O affects barrier function and induces chloride secretion in HT-29/B6 colon epithelial cells. Am J Physiol Gastrointest Liver Physiol 296: G1350-G1359, 2009. First published April 16, 2009; doi:10.1152/ajpgi.00040.2009.-Listeria monocytogenes is a food-borne pathogen, which is able to induce diarrhea when residing in the intestine. We studied the effect of listeriolysin O (LLO), an extracellular virulence factor of L. monocytogenes, on intestinal transport and barrier function in monolayers of HT-29/B6 human colon cells using the Ussing technique to understand the pathomechanisms involved. Mucosal addition of LLO, but not a LLO mutant, induced a dose-and pH-dependent increase in short-circuit current (I-SC). Sodium and chloride tracer flux and DIDS sensitivity studies revealed that I-SC was mainly due to electrogenic chloride secretion. Barrier function was impaired by LLO, as assessed by transepithelial resistance (R-t) and mannitol flux measurements. Intracellular signal transduction occurred through Ca2+ release from intracellular stores and PKC activation. In conclusion, listeriolysin induces chloride secretion and perturbs epithelial barrier function, thus potentially contributing to Listeria-induced diarrhea.
Zitierstile
Harvard-Zitierstil: Richter, J., Gitter, A., Guenzel, D., Weiss, S., Mohamed, W., Chakraborty, T., et al. (2009) Listeriolysin O affects barrier function and induces chloride secretion in HT-29/B6 colon epithelial cells, American Journal of Physiology - Gastrointestinal and Liver Physiology, 296(6), pp. G1350-G1359. https://doi.org/10.1152/ajpgi.00040.2009
APA-Zitierstil: Richter, J., Gitter, A., Guenzel, D., Weiss, S., Mohamed, W., Chakraborty, T., Fromm, M., & Schulzke, J. (2009). Listeriolysin O affects barrier function and induces chloride secretion in HT-29/B6 colon epithelial cells. American Journal of Physiology - Gastrointestinal and Liver Physiology. 296(6), G1350-G1359. https://doi.org/10.1152/ajpgi.00040.2009
Schlagwörter
ADHESION MOLECULES; CHOLERA-TOXIN; CHOLESTEROL-DEPENDENT CYTOLYSIN; GASTROENTERITIS; HT-29/B6 cells; MONOCYTOGENES; transepithelial resistance
