Journal article

Publication year: 2009

Pages: 730-739

Journal: Intensive Care Medicine

Volume number: 35

Issue number: 4

ISSN: 0342-4642

eISSN: 1432-1238

Open access status: Hybrid

DOI Link: https://doi.org/10.1007/s00134-009-1394-7

Publisher: Springer

Abstract: 
Purpose: We studied gene expression differences in brain homogenate, hippocampus, somatosensory cortex and cerebellum of rats suffering from sepsis-associated delirium and analyzed the effects of norepinephrine and 1,400 W (specific inhibitor of the inducible nitric-oxide synthase). Methods: We applied microarray screenings to rat brain homogenate 1, 3 and 4.5 h after lipopolysaccharide (LPS, 5 mg/kg) or 0.9% NaCl treatment. Therapy groups were analyzed after 4.5 h. Validations and compartment specific investigations were carried out by real-time PCR. Results: Most striking gene expression differences were seen 4.5 h after LPS administration, especially within the hippocampus (chemokines and endothelial cell-specific molecule 1). Norepinephrine resulted in a discrete chemokine upregulation, while 1,400 W had hardly any effect. Conclusion: Strongest gene regulations were found within the hippocampus. Norepinephrine showed a tendency of having a proinflammatory influence, while 1,400 W had no clear-cut effect onto the gene expression level.

Harvard Citation style: Wolff, S., Klatt, S., Wolff, J., Wilhelm, J., Fink, L., Kaps, M., et al. (2009) Endotoxin-induced gene expression differences in the brain and effects of iNOS inhibition and norepinephrine, Intensive Care Medicine, 35(4), pp. 730-739. https://doi.org/10.1007/s00134-009-1394-7

APA Citation style: Wolff, S., Klatt, S., Wolff, J., Wilhelm, J., Fink, L., Kaps, M., & Rosengarten, B. (2009). Endotoxin-induced gene expression differences in the brain and effects of iNOS inhibition and norepinephrine. Intensive Care Medicine. 35(4), 730-739. https://doi.org/10.1007/s00134-009-1394-7