Journal article

Publication year: 2009

Pages: 77-83

Journal: Cardiovascular Research

Volume number: 82

Issue number: 1

ISSN: 0008-6363

eISSN: 1755-3245

DOI Link: https://doi.org/10.1093/cvr/cvp027

Publisher: Oxford University Press

Abstract: 

In patients with congestive heart failure, plasma parathyroid hormone (PTH) levels are positively associated with cardiac function. PTH, used to mobilize stem cells from the bone marrow after myocardial infarction, causes an increased left ventricular ejection fraction. The aim of this study was to investigate whether low but plasma-relevant concentrations of PTH directly influence the contractile properties of cardiomyocytes.

Isolated adult rat ventricular cardiomyocytes were exposed to PTH(1-34) or full-length PTH at picomolar concentrations for 24 h. Cell shortening was measured at 2 Hz as a cellular correlate of inotropic responsiveness. Intracellular calcium was measured in Fura-AM-loaded cells. PTH(1-3) (20-200 pM) and full-length PTH (200 pM) increased cell shortening within 24 h. PTH had no effect on cell size, but resting and peak systolic calcium concentrations were elevated. The beneficial effect of PTH was mediated via its cAMP/protein kinase A-activating domain and attenuated by addition of a protein kinase A inhibitor. In contrast, PTH peptides representing a protein kinase C-activating domain but not a cAMP/protein kinase A-activating domain or peptides that represent none of these domains had no effect on cell shortening. The effect of PTH on cell shortening was strong at low concentrations of extracellular calcium but declined at higher calcium concentrations. PTH downregulated the expression of the calcium sensing receptor, a receptor known to antagonize the action of PTH on calcium transport. Furthermore, PTH antagonized the angiotensin II-induced loss of cell function.

Low concentrations of PTH improve cell shortening by increasing calcium load at rest. By this mechanism cardiomyocytes compensate reduced extracellular calcium levels as they occur in patients with heart failure.

Harvard Citation style: Tastan, I., Schreckenberg, R., Mufti, S., Abdallah, Y., Piper, H. and Schlueter, K. (2009) Parathyroid hormone improves contractile performance of adult rat ventricular cardiomyocytes at low concentrations in a non-acute way, Cardiovascular Research, 82(1), pp. 77-83. https://doi.org/10.1093/cvr/cvp027

APA Citation style: Tastan, I., Schreckenberg, R., Mufti, S., Abdallah, Y., Piper, H., & Schlueter, K. (2009). Parathyroid hormone improves contractile performance of adult rat ventricular cardiomyocytes at low concentrations in a non-acute way. Cardiovascular Research. 82(1), 77-83. https://doi.org/10.1093/cvr/cvp027