Journal article

Novel bacterial artificial chromosome vector pUvBBAC for use in studies of the functional genomics of Listeria spp.


Authors list: Hain, Torsten; Otten, Sonja; von Both, Ulrich; Chatterjee, Som S.; Technow, Ulrike; Billion, Andre; Ghai, Rohit; Mohamed, Walid; Domann, Eugen; Chakraborty, Trinad

Publication year: 2008

Pages: 1892-1901

Journal: Applied and Environmental Microbiology

Volume number: 74

Issue number: 6

ISSN: 0099-2240

eISSN: 1098-5336

Open access status: Green

DOI Link: https://doi.org/10.1128/AEM.00415-07

Publisher: American Society for Microbiology


Abstract: 
Bacterial artificial chromosome (BAC) vectors are important tools for microbial genome research. We constructed a novel BAC vector, pUvBBAC, for replication in both gram-negative and gram-positive bacterial hosts. The pUvBBAC vector was used to generate a BAC library for the facultative intracellular pathogen Listeria monocytogenes EGD-e. The library had insert sizes ranging from 68 to 178 kb. We identified two recombinant BACs from the L. monocytogenes pUvBBAC library that each contained the entire virulence gene cluster (vgc) of L. monocytogenes and transferred them to a nonpathogenic Listeria innocua strain. Recombinant L. innocua strains harboring pUvBBAC+vgcl and pUvBBAC+vgc2 produced the vgc-specific listeriolysin (LLO) and actin assembly protein ActA and represent the first reported cloning of the vgc locus in its entirety. The use of the novel broad-host-range BAC vector pUvBBAC extends the versatility of this technology and provides a powerful platform for detailed functional genomics of gram-positive bacteria as well as its use in explorative functional metagenomics.



Citation Styles

Harvard Citation style: Hain, T., Otten, S., von Both, U., Chatterjee, S., Technow, U., Billion, A., et al. (2008) Novel bacterial artificial chromosome vector pUvBBAC for use in studies of the functional genomics of Listeria spp., Applied and Environmental Microbiology, 74(6), pp. 1892-1901. https://doi.org/10.1128/AEM.00415-07

APA Citation style: Hain, T., Otten, S., von Both, U., Chatterjee, S., Technow, U., Billion, A., Ghai, R., Mohamed, W., Domann, E., & Chakraborty, T. (2008). Novel bacterial artificial chromosome vector pUvBBAC for use in studies of the functional genomics of Listeria spp.. Applied and Environmental Microbiology. 74(6), 1892-1901. https://doi.org/10.1128/AEM.00415-07



Keywords

BAC LIBRARY; HUMAN DNA; MAGNAPORTHE-GRISEA; PHYSICAL MAP; PLASMID COPY NUMBER

Last updated on 2025-10-06 at 09:44