Journalartikel

Jahr der Veröffentlichung: 2007

Seiten: 23603-23612

Zeitschrift: Journal of Biological Chemistry

Bandnummer: 282

Heftnummer: 32

eISSN: 1083-351X

Open Access Status: Hybrid

DOI Link: https://doi.org/10.1074/jbc.M701120200

Verlag: Elsevier

Abstract: 
Human neutrophil-specific CD177 (NB1 and PRV-1) has been reported to be up-regulated in a number of inflammatory settings, including bacterial infection and granulocyte-colonystimulating factor application. Little is known about its function. By flow cytometry and immunoprecipitation studies, we identified platelet endothelial cell adhesion molecule-1 (PECAM-1) as a binding partner of CD177. Real-time protein-protein analysis using surface plasmon resonance confirmed a cation-dependent, specific interaction between CD177 and the heterophilic domains of PECAM-1. Monoclonal antibodies against CD177 and against PECAM-1 domain 6 inhibited adhesion of U937 cells stably expressing CD177 to immobilized PECAM-1. Transendothelial migration of human neutrophils was also inhibited by these antibodies. Our findings provide direct evidence that neutrophil-specific CD177 is a heterophilic binding partner of PECAM-1. This interaction may constitute a new pathway that participates in neutrophil transmigration.

Harvard-Zitierstil: Sachs, U., Andrei-Selmer, C., Maniar, A., Weiss, T., Paddock, C., Orlova, V., et al. (2007) The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31), Journal of Biological Chemistry, 282(32), pp. 23603-23612. https://doi.org/10.1074/jbc.M701120200

APA-Zitierstil: Sachs, U., Andrei-Selmer, C., Maniar, A., Weiss, T., Paddock, C., Orlova, V., Choi, E., Newman, P., Preissner, K., Chavakis, T., & Santoso, S. (2007). The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31). Journal of Biological Chemistry. 282(32), 23603-23612. https://doi.org/10.1074/jbc.M701120200