Conference paper

Publication year: 2007

Pages: 2290-2293

Journal: Life Sciences

Volume number: 80

Issue number: 24-25

ISSN: 0024-3205

eISSN: 1879-0631

DOI Link: https://doi.org/10.1016/j.lfs.2007.01.024

Conference: 2nd International Symposium on Non-Neuronal Acetylcholine

Publisher: Elsevier

Abstract: 
Administration of recombinant human keratinocyte growth factor (rHuKGF, Delta 23N-KGF, palifermin) protects the lung against a variety of injurious stimuli. The exact mechanisms leading to lung protection are unknown. Alterations in the non-neuronal cholinergic system of the lung might be involved, as vital pulmonary functions are regulated by acetylcholine. Here, we investigated the effect of KGF on the expression of nicotinic acetylcholine receptor subunits alpha 7, alpha 9 and alpha 10 in rat lungs. Adult rats were treated via intratracheal instillation with rHuKGF or with an equivalent volume of PBS. The expression of nicotinic acetylcholine receptor subunits was analyzed by real-time RT-PCR, immunoblotting and immunohistochemistry. Treatment with rHuKGF led to a decreased expression of nicotinic receptor subunit alpha 7 in the total lung. In contrast, the expression of the receptor subunits alpha 9 and alpha 10 was up-regulated. In conclusion, nicotinic acetylcholine receptors are differentially regulated by KGF treatment in vivo, which might result in changes in the biological effects of acetylcholine. (c) 2007 Published by Elsevier Inc.

Harvard Citation style: Grau, V., Wilker, S., Hartmann, P., Lips, K., Grando, S., Padberg, W., et al. (2007) Administration of keratinocyte growth factor (KGF) modulates the pulmonary expression of nicotinic acetylcholine receptor subunits α7, α9 and α10, Life Sciences, 80(24-25), pp. 2290-2293. https://doi.org/10.1016/j.lfs.2007.01.024

APA Citation style: Grau, V., Wilker, S., Hartmann, P., Lips, K., Grando, S., Padberg, W., Fehrenbach, H., & Kummer, W. (2007). Administration of keratinocyte growth factor (KGF) modulates the pulmonary expression of nicotinic acetylcholine receptor subunits α7, α9 and α10. Life Sciences. 80(24-25), 2290-2293. https://doi.org/10.1016/j.lfs.2007.01.024