Journal article

Publication year: 2007

Pages: 41-49

Journal: Medical Mycology

Volume number: 45

Issue number: 1

ISSN: 1369-3786

eISSN: 1460-2709

Open access status: Bronze

DOI Link: https://doi.org/10.1080/13693780601003827

Publisher: Oxford University Press

Abstract: 
The aim of the study was the characterization of Malassezia pachydermatis and its pigment-producing subgroup using biochemical tests and RAPD. It was of interest to determine whether particular RAPID patterns could be used to indicate pigment production, as well as a close genetic relatedness to Malassezia furfur. Therefore, 210 strains of M. pachydermatis were examined for morphology, catalase and beta-glucosidase activity, lipid and carbohydrate assimilation and the tryptophan-dependent synthesis of pigments. Of these, 114 strains were subjected to RAPD analyses. A multivarlate logistic regression model was applied to classify M. pachydermatis isolates regarding their pigment production by using genetic and biological parameters. Biological and RAPID findings showed a high biological and genetic diversity within the species M. pachydermatis and within pigment producers. RAPID analysis revealed 28 genotypes within 114 strains tested. Pigment producing strains could not be assigned to a common RAPD profile, but a genetic relatedness of pigment-producing M. pachydermatis with M. furfur can be assumed. A particular RAPID pattern allowed statistically significant probability of pigment production (P < 0.001) and might be used as a tool to rapidly detect pigment producing M. pachydermatis, e.g. in Malassezia-associated pityriasis versicolor. The reported method is useful for identification of pigment producing M. pachlydermatis isolates and has advantages over established tests.

Harvard Citation style: Hossain, H., Landgraf, V., Weiss, R., Mann, M., Hayatpour, J., Chakraborty, T., et al. (2007) Genetic and biochemical characterization of Malassezia pachydermatis with particular attention to pigment-producing subgroups, Medical Mycology, 45(1), pp. 41-49. https://doi.org/10.1080/13693780601003827

APA Citation style: Hossain, H., Landgraf, V., Weiss, R., Mann, M., Hayatpour, J., Chakraborty, T., & Mayser, P. (2007). Genetic and biochemical characterization of Malassezia pachydermatis with particular attention to pigment-producing subgroups. Medical Mycology. 45(1), 41-49. https://doi.org/10.1080/13693780601003827