Conference paper

Publication year: 2007

Pages: 255-261

Journal: European Urology

Volume number: 51

Issue number: 1

ISSN: 0302-2838

DOI Link: https://doi.org/10.1016/j.eururo.2006.05.002

Conference: 100th Annual Meeting of the American-Urological-Association

Publisher: Elsevier

Abstract: 

Objectives: The development of fibrotic diseases is associated with alterations in the transforming growth factor beta (TGF-beta) pathway. We have investigated the expression and activity of Smad transcription factors of the TGF-beta pathway in primary tunical fibroblasts derived from patients with Peyronie's disease and from controls.

Methods: Primary fibroblasts were established from biopsies obtained from plaques of 16 patients with Peyronie's disease or the tunica albuginea of 8 control patients. The expression and activity of Smad transcription factors in control and TGF-beta-stimulated primary fibroblasts were investigated at the RNA and protein level by reverse transcription-polymerase chain reaction, Western blotting, and immunofluorescence.

Results: RNA expression levels of Smad3 and Smad4 were significantly increased in fibroblasts from patients with Peyronie's disease. When stimulated with TGF-beta 1, fibroblasts showed rapid nuclear translocation of Smad2/3, as soon as 15 min after stimulation. This effect was more pronounced and exhibited an earlier onset in fibroblasts from patients with Peyronie's disease, compared with controls. In addition, an increased nuclear retention time of Smad4 was observed in fibroblasts from patients with Peyronie's disease.

Conclusions: The expression and activity of Smad transcription factors of the TGF-beta pathway is increased in fibroblasts of patients with Peyronie's disease. Alterations in the TGF-beta pathway seem to be a pathogenetic factor in the development of Peyronie's disease. (c) 2006 European Association of Urology. Published by Elsevier B.V. All rights reserved.

Harvard Citation style: Haag, S., Hauck, E., Szardening-Kirchner, C., Diemer, T., Cha, E., Weidner, W., et al. (2007) Alterations in the transforming growth factor (TGF)-β pathway as a potential factor in the pathogenesis of Peyronie's disease, European Urology, 51(1), pp. 255-261. https://doi.org/10.1016/j.eururo.2006.05.002

APA Citation style: Haag, S., Hauck, E., Szardening-Kirchner, C., Diemer, T., Cha, E., Weidner, W., & Eickelberg, O. (2007). Alterations in the transforming growth factor (TGF)-β pathway as a potential factor in the pathogenesis of Peyronie's disease. European Urology. 51(1), 255-261. https://doi.org/10.1016/j.eururo.2006.05.002