Journal article

Publication year: 2006

Pages: 651-657

Journal: Biochemical and Biophysical Research Communications

Volume number: 351

Issue number: 3

ISSN: 0006-291X

eISSN: 1090-2104

DOI Link: https://doi.org/10.1016/j.bbrc.2006.10.083

Publisher: Elsevier

Abstract: 
Transforming growth factor (TGF)-beta ligands signal through transmembrane type I and type 11 serine/threonine kinase receptors, which form heteromeric signalling complexes upon ligand binding. Type 11 TGF-beta receptors (T beta RII) are reported to exist as homodimers at the cell surface, but the oligomerization pattern and dynamics of T beta RII splice variants in live cells has not been demonstrated thus far. Using co-immunoprecipitation and bioluminescence resonance energy transfer (BRET), we demonstrate that the mouse T beta RII receptor splice variant T beta RII-B is capable of forming ligand-independent homodimers and heterodimers with T beta RII. The homomeric interaction of mouse (m)T beta RII-B isoforms, however, is less robust than the heteromeric interactions of mT beta RII-B with wild-type T beta RII, which indicates that these receptors may be more likely to heterodimerize when both receptors are expressed. Moreover, we demonstrate that mT beta RII-B is a signalling receptor with ubiquitous tissue expression. Our study thus demonstrates previously unappreciated complex formation of TGF-beta type 11 receptors, and suggests that mT beta RII-B can direct TGF-beta-induced signalling in vitro and in vivo. (c) 2006 Elsevier Inc. All rights reserved.

Harvard Citation style: Krishnaveni, M., Hansen, J., Seeger, W., Morty, R., Sheikh, S. and Eickelberg, O. (2006) Constitutive homo- and hetero-oligomerization of TβRII-B, an alternatively spliced variant of the mouse TGF-β type II receptor, Biochemical and Biophysical Research Communications, 351(3), pp. 651-657. https://doi.org/10.1016/j.bbrc.2006.10.083

APA Citation style: Krishnaveni, M., Hansen, J., Seeger, W., Morty, R., Sheikh, S., & Eickelberg, O. (2006). Constitutive homo- and hetero-oligomerization of TβRII-B, an alternatively spliced variant of the mouse TGF-β type II receptor. Biochemical and Biophysical Research Communications. 351(3), 651-657. https://doi.org/10.1016/j.bbrc.2006.10.083