Journal article

Publication year: 2006

Pages: 378-386

Journal: Archives of Microbiology

Volume number: 184

Issue number: 6

ISSN: 0302-8933

eISSN: 1432-072X

DOI Link: https://doi.org/10.1007/s00203-005-0056-6

Publisher: Springer

Abstract: 
In this communication, we present a genetic analysis of the glnN promoter region of Synechococcus elongatus PCC 7942. luxAB reporter fusions were used to characterize the glnN promoter by deletion and site-directed mutational analysis. Reporter gene expression analysis was performed in S. elongatus wild-type and mutant strains to reveal the role of the global nitrogen responsive transcription factor NtcA and of the P-II signalling protein on regulation of glnN gene expression. A non-canonical NtcA-binding motif is responsible for NtcA-dependent, nitrogen-responsive regulation of glnN. The P-II signalling protein has opposing effects on NtcA-dependent glnN expression. Under conditions of nitrate-growth, it depresses expression, whereas under conditions of combined nitrogen starvation, it is required for full induction. Furthermore, sequences upstream of the NtcA-binding site have repressive effect on glnN promoter activity.

Harvard Citation style: Aldehni, M. and Forchhammer, K. (2006) Analysis of a non-canonical NtcA-dependent promoter in Synechococcus elongatus and its regulation by NtcA and P_II, Archives of Microbiology, 184(6), pp. 378-386. https://doi.org/10.1007/s00203-005-0056-6

APA Citation style: Aldehni, M., & Forchhammer, K. (2006). Analysis of a non-canonical NtcA-dependent promoter in Synechococcus elongatus and its regulation by NtcA and P_II. Archives of Microbiology. 184(6), 378-386. https://doi.org/10.1007/s00203-005-0056-6