Journal article

Publication year: 2006

Pages: 484-490

Journal: The Journal of Immunology

Volume number: 176

Issue number: 1

ISSN: 0022-1767

eISSN: 1550-6606

DOI Link: https://doi.org/10.4049/jimmunol.176.1.484

Publisher: American Association of Immunologists

Abstract: 
Nucleotide-binding oligomerization domain (Nod) proteins serve as intracellular pattern recognition molecules recognizing peptidoglycans. To further examine intracellular immune recognition, we used Listeria monocytogenes as an organism particularly amenable for studying innate immunity to intracellular pathogens. In contrast to wild-type L. monocytogenes, the nonpathogenic Listeria innocua, or L. monocytogenes mutants lacking internalin B or listeriolysin O, poorly invaded host cells and escaped into host cell cytoplasm, respectively, and were therefore used as controls. In this study, we show that only the invasive wild-type L. monocytogenes, but not the listeriolysin O- or internalin B-negative L. monocytogenes mutants or L. innocua, substantially induced IL-8 production in HUVEC. RNA interference and Nod1-overexpression experiments demonstrated that Nod1 is critically involved in chemokine secretion and NF-kappa B activation initiated by L. monocytogenes in human endothelial cells. Moreover, we show for the first time that Nod1 mediated activation of p38 MAPK signaling induced by L. monocytogenes. Finally, L. monocytogenes and Nod1-induced IL-8 production was blocked by a specific p38 inhibitor. In conclusion, L monocytogenes induced a Nod1-dependent activation of p38 MAPK signaling and NF-kappa B which resulted in IL-8 production in endothelial cells. Thus, Nod1 is an important component of a cytoplasmic surveillance pathway.

Harvard Citation style: Opitz, B., Püschel, A., Beermann, W., Hocke, A., Föster, S., Schmeck, B., et al. (2006) Listeria monocytogenes activated p38 MAPK and induced IL-8 secretion in a nucleotide-binding oligomerization domain 1-dependent manner in endothelial cells, The Journal of Immunology, 176(1), pp. 484-490. https://doi.org/10.4049/jimmunol.176.1.484

APA Citation style: Opitz, B., Püschel, A., Beermann, W., Hocke, A., Föster, S., Schmeck, B., van Laak, V., Chakraborty, T., Suttorp, N., & Hippenstiel, S. (2006). Listeria monocytogenes activated p38 MAPK and induced IL-8 secretion in a nucleotide-binding oligomerization domain 1-dependent manner in endothelial cells. The Journal of Immunology. 176(1), 484-490. https://doi.org/10.4049/jimmunol.176.1.484