Journal article

Publication year: 2005

Pages: 2489-2499

Journal: Life Sciences

Volume number: 77

Issue number: 20

ISSN: 0024-3205

eISSN: 1879-0631

DOI Link: https://doi.org/10.1016/j.lfs.2004.12.053

Publisher: Elsevier

Abstract: 

Voltage-dependent Ca2+ channels of fura-2-loaded ganglionic cells from the myenteric plexus of newborn rats were pharmacologically characterised. In contrast to completely dissociated myenteric cells, intact ganglia showed a stronger loading with the Ca2+-sensitive dye and a reproducible stimulation of the fura-2 signal by the cholinergic agonist, carbachol. A depolarisation-induced increase in the intracellular Ca2+ concentration ([Ca2+](i)) was induced by superfusion with 35 mmol l(-1) KCl. This increase in [Ca2+](i) was sensitive to Ni2+ and CO2+ as well as omega-conotoxin MVIIA, omega-agatoxin IVA, and SNX-482. The strongest inhibition was achieved by nifedipine (5 x 10(-7) mol l(-1)) and omega-conotoxin GVIA (4.3 x 10(-7) Mol l(-1)). These two blockers also inhibited the [Ca2+](i) increase evoked by nicotinic receptor stimulation. Consequently, isolated myenteric ganglia in culture express different types of voltage-dependent Ca2+ channels, from which the L- and the N-type seem to be the most important. When exposed to mediators of inflammation such as tumor necrosis factor-alpha (TNF-alpha) or different prostaglandins, no pronounced alterations in the fura-2 ratio were observed suggesting that changes in the Ca2+-signalling are not centrally involved in the response of enteric ganglionic cells to these paracrine substances.

Harvard Citation style: Schäufele, N. and Diener, M. (2005) Pharmacological characterisation of voltage-dependent Ca2+ channels in isolated ganglia from the myenteric plexus, Life Sciences, 77(20), pp. 2489-2499. https://doi.org/10.1016/j.lfs.2004.12.053

APA Citation style: Schäufele, N., & Diener, M. (2005). Pharmacological characterisation of voltage-dependent Ca2+ channels in isolated ganglia from the myenteric plexus. Life Sciences. 77(20), 2489-2499. https://doi.org/10.1016/j.lfs.2004.12.053