Journalartikel

Jahr der Veröffentlichung: 2005

Seiten: G586-G591

Zeitschrift: American Journal of Physiology - Gastrointestinal and Liver Physiology

Bandnummer: 289

Heftnummer: 3

ISSN: 0193-1857

eISSN: 1522-1547

DOI Link: https://doi.org/10.1152/ajpgi.00020.2005

Verlag: American Physiological Society

Abstract: 
Intact myenteric ganglia from 4- to 10-day-old rats were isolated from the small intestine. The preparations were cultured overnight, and drugs were applied within this time frame (20 h). Whole cell patch-clamp technique was used to measure basal membrane potential and carbachol-induced depolarization at neurons within these ganglia. Pretreatment with TNF-alpha (100 ng/ml) hyperpolarized the membrane (from -31.0 +/- 2.7 mV under control conditions to -61.2 +/- 3.2 mV in the presence of the cytokine) and potentiated the depolarization induced by carbachol (from 5.2 +/- 0.7 mV under control conditions to 27.5 +/- 2.0 mV in the presence of the cytokine). These effects were mimicked by carbocyclic thromboxane A(2) (10(-6) mol/l), a stable thromboxane A(2) agonist. The TNF-alpha action was inhibited by 1-benzylimidazole (2 x 10(-4) mol/l), a thromboxane synthase inhibitor, and BAY U 3405 (5 x 10(-4) mol/l), an inhibitor of thromboxane receptors. Measurements of thromboxane production in the supernatant of the culture revealed an increased concentration of thromboxane B-2, the stable metabolite of thromboxane A2, after exposure to TNF-alpha. Immuncytochemical staining for cyclooxygenase-2 (COX-2) and the neuronal marker microtubule-associating protein-2 revealed an upregulation of COX-2 in myenteric neurons after exposure to the cytokine. These results demonstrate the involvement of COX-2 and the subsequent production of thromboxane A(2) in the presence of TNF-alpha.

Harvard-Zitierstil: Rehn, M., Hild, D. and Diener, M. (2005) Upregulation of cyclooxygenase-2 and thromboxane A2 production mediate the action of tumor necrosis factor-α in isolated rat myenteric ganglia, American Journal of Physiology - Gastrointestinal and Liver Physiology, 289(3), pp. G586-G591. https://doi.org/10.1152/ajpgi.00020.2005

APA-Zitierstil: Rehn, M., Hild, D., & Diener, M. (2005). Upregulation of cyclooxygenase-2 and thromboxane A2 production mediate the action of tumor necrosis factor-α in isolated rat myenteric ganglia. American Journal of Physiology - Gastrointestinal and Liver Physiology. 289(3), G586-G591. https://doi.org/10.1152/ajpgi.00020.2005