Journal article

Publication year: 2004

Pages: 980-984

Journal: British Journal of Cancer

Volume number: 91

Issue number: 5

ISSN: 0007-0920

Open access status: Hybrid

DOI Link: https://doi.org/10.1038/sj.bjc.6602033

Publisher: Springer Nature [academic journals on nature.com]

Abstract: 
Proliferating cells, especially tumour cells, express a special isoenzyme of pyruvate kinase, termed M2-PK, which can occur in a tetrameric form with a high affinity to its substrate, phosphoenolpyruvate (PEP), and in a dimeric form with a low PEP affinity. In tumour cells, the dimeric form is usually predominant and is therefore termed Tumour M2-PK. The levels of Tumour M2-PK within tumours and in EDTA-plasma correlate with staging and the ability of the tumour cells to metastasise. Since most colorectal tumours grow intraluminally, it appeared interesting to determine whether Tumour M2-PK is detectable in the faeces of tumour patients. Stool samples were tested by ELISA from controls without colorectal cancer and colorectal cancer patients. Whereas Tumour M2-PK levels were low in the control group (mean value+/-s.e.m.: 3.3+/-0.4, n=144), they were high in the case of colorectal cancer (56.1+/-15.3, n=60). At a cutoff value of 4 U ml(-1), the sensitivity was 73%. TNM and Dukes' classification of the tumours revealed a strong correlation between faecal Tumour M2-PK levels and staging. The determination of Tumour M2-PK in faeces provides a new promising screening tool for colorectal tumours.

Harvard Citation style: Hardt, P., Mazurek, S., Toepler, M., Schlierbach, P., Bretzel, R., Eigenbrodt, E., et al. (2004) Faecal tumour M2 pyruvate kinase: a new, sensitive screening tool for colorectal cancer, British Journal of Cancer, 91(5), pp. 980-984. https://doi.org/10.1038/sj.bjc.6602033

APA Citation style: Hardt, P., Mazurek, S., Toepler, M., Schlierbach, P., Bretzel, R., Eigenbrodt, E., & Kloer, H. (2004). Faecal tumour M2 pyruvate kinase: a new, sensitive screening tool for colorectal cancer. British Journal of Cancer. 91(5), 980-984. https://doi.org/10.1038/sj.bjc.6602033