Journal article

Publication year: 2004

Pages: 32-42

Journal: Autonomic Neuroscience: Basic & Clinical

Volume number: 113

Issue number: 1-2

ISSN: 1566-0702

eISSN: 1872-7484

DOI Link: https://doi.org/10.1016/j.autneu.2004.05.008

Publisher: Elsevier

Abstract: 
Stimulation of nicotinic acetylcholine receptors (nAChR) excites peripheral sensory nerve fibres, but also exert antinociceptive effects. The differences in these nAChR-mediated effects could be related to the expression of different nAChR subtypes located on nociceptive neurons. In the present study, we focused on the recently described alpha10-nAChR subunit, and on alpha4 and alpha7 subunits, which are the most abundant subunits in the central nervous system. In nociceptive neurons from thoracic and lumbar dorsal root ganglia (DRG), nAChR subunits were found at transcriptional (RT-PCR), translational (immunohistochemistry) and functional levels. Cultured DRG neurons express mRNA for the subunits alpha2-7 and alpha10. The alpha-subunit proteins 4, 7 and 10 were colocalised in virtually all nociceptive neurons that were identified by immunoreactivity for the vanilloid receptor TRPV-1. These findings were corroborated by current recordings and calcium measurements, which revealed excitatory inward currents and calcium responses in capsaicin sensitive neurons. (C) 2004 Elsevier B.V. All rights reserved.

Harvard Citation style: Haberberger, R., Bernardini, N., Kress, M., Hartmann, P., Lips, K. and Kummer, W. (2004) Nicotinic acetylcholine receptor subtypes in nociceptive dorsal root ganglion neurons of the adult rat, Autonomic Neuroscience: Basic & Clinical, 113(1-2), pp. 32-42. https://doi.org/10.1016/j.autneu.2004.05.008

APA Citation style: Haberberger, R., Bernardini, N., Kress, M., Hartmann, P., Lips, K., & Kummer, W. (2004). Nicotinic acetylcholine receptor subtypes in nociceptive dorsal root ganglion neurons of the adult rat. Autonomic Neuroscience: Basic & Clinical. 113(1-2), 32-42. https://doi.org/10.1016/j.autneu.2004.05.008