Journalartikel

Jahr der Veröffentlichung: 2004

Seiten: 32-35

Zeitschrift: Neuroscience Letters

Bandnummer: 361

Heftnummer: 1-3

ISSN: 0304-3940

eISSN: 1872-7972

DOI Link: https://doi.org/10.1016/S0304-3940(03)01073-5

Verlag: Elsevier

Abstract: 
The contribution of nicotinic acetylcholine receptors (nAChRs) to stimulation of NO-production was investigated in isolated rat dorsal root ganglion (DRG) neurons utilizing an NO-sensitive fluorescent indicator 4,5-diaminofluorescein-diacetate (DAF-2DA) and appropriate channel blockers. RT-PCR and immunohistochemical analysis of NOS isoforms in cultured neurons revealed the expression of eNOS in the vast majority of neurons, nNOS in about 5-10%, and iNOS only exceptionally. Application of nicotine resulted in an abrupt increase in DAF-2T fluorescence in 65% of neuronal cell bodies that was fully sensitive to the general nAChR antagonist mecamylamine. Methyllycaconitine reduced the number of nicotine-sensitive neurons and the extent of NO-generation. Thus, alpha7- and/or alpha9/10-nAChRs are required for nicotine-induced NO-production in a subpopulation of DRG neurons, and appear to be partially involved in the remaining, larger subpopulation. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

Harvard-Zitierstil: Papadopolou, S., Hartmann, P., Lips, K., Kummer, W. and Haberberger, R. (2004) Nicotinic receptor mediated stimulation of NO-generation in neurons of rat thoracic dorsal root ganglia, Neuroscience Letters, 361(1-3), pp. 32-35. https://doi.org/10.1016/S0304-3940(03)01073-5

APA-Zitierstil: Papadopolou, S., Hartmann, P., Lips, K., Kummer, W., & Haberberger, R. (2004). Nicotinic receptor mediated stimulation of NO-generation in neurons of rat thoracic dorsal root ganglia. Neuroscience Letters. 361(1-3), 32-35. https://doi.org/10.1016/S0304-3940(03)01073-5