Journal article

Publication year: 2004

Pages: 100-105

Journal: Toxicologic Pathology

Volume number: 32

Issue number: 1

ISSN: 0192-6233

eISSN: 1533-1601

DOI Link: https://doi.org/10.1080/01926230490266000

Publisher: SAGE Publications

Abstract: 
To determine the lobular distribution of hepatocellular proliferation, S-phase response was measured in untreated adult male B6C3F1 and C57BL mice at ages 11, 14, and 23 weeks. The percentage of cells in S-phase (labeling index, LI) was evaluated using immunohistochemical detection of 5-bromo-2'-deoxyuridine (BrdU). The BrdU was delivered either by a single ip injection 2 hr prior to sacrifice or via an osmotic minipump implanted subcutaneously for 3 or 7 days. Mitotic and apoptotic indices were determined on H&E stained sections. Animals of both strains and all ages revealed highest LI in the intermediate compartment of the liver acinus (zone 2) irrespective of the length of BrdU application. Cell proliferation in this zone was at all time points significantly higher than elsewhere in the liver. The mitotic index confirmed the data obtained by the S-phase response study. Apoptosis was rarely observed. With regard to rodent data in chemical carcinogenesis and the way they should be evaluated, this study proved that the acinar distribution of proliferating cells in liver of mice is different from that in rats, since, according to the literature, rats reveal highest cell proliferative activity in the periportal zone.

Harvard Citation style: Burkhardt, S., Bahnemann, R., Failing, K. and Reinacher, M. (2004) Zonal distribution of cell proliferation in the liver of untreated B6C3F1 and C57BL mice, Toxicologic Pathology, 32(1), pp. 100-105. https://doi.org/10.1080/01926230490266000

APA Citation style: Burkhardt, S., Bahnemann, R., Failing, K., & Reinacher, M. (2004). Zonal distribution of cell proliferation in the liver of untreated B6C3F1 and C57BL mice. Toxicologic Pathology. 32(1), 100-105. https://doi.org/10.1080/01926230490266000