Journalartikel

Jahr der Veröffentlichung: 2003

Seiten: 383-392

Zeitschrift: Journal of Chromatography B

Bandnummer: 789

Heftnummer: 2

ISSN: 1570-0232

eISSN: 1873-376X

DOI Link: https://doi.org/10.1016/S1570-0232(03)00163-6

Verlag: Elsevier

Abstract: 
For the first time, a procedure is described for the quantitative analysis of free alpha-keto acid content in human neutrophils (PMNs) relative to single cell number by reversed-phase fluorescence high-performance liquid chromatography. The procedure is minimally invasive and is unsurpassed in the quality of PMN separation, ease of sample preparation as well as sample stability. This method can satisfy the rigorous demands for an ultra-sensitive, comprehensive and rapid intracellular a-keto acid analysis in particularly for the surveillance of severe diseases as well as cellular or organ dysfunction. (C) 2003 Elsevier Science B.V.. All rights reserved.

Harvard-Zitierstil: Mühling, J., Fuchs, M., Campos, M., Gonter, J., Engel, J., Sablotzki, A., et al. (2003) Quantitative determination of free intracellular α-keto acids in neutrophils, Journal of Chromatography B, 789(2), pp. 383-392. https://doi.org/10.1016/S1570-0232(03)00163-6

APA-Zitierstil: Mühling, J., Fuchs, M., Campos, M., Gonter, J., Engel, J., Sablotzki, A., Menges, T., Weiss, S., Dehne, M., Krüll, M., & Hempelmann, G. (2003). Quantitative determination of free intracellular α-keto acids in neutrophils. Journal of Chromatography B. 789(2), 383-392. https://doi.org/10.1016/S1570-0232(03)00163-6