Conference paper

Development and evaluation of an HPLC-DAD method for determination of synthetic food colorants


Authors listKirschbaum, J; Krause, C; Pfalzgraf, S; Brückner, H

Publication year2003

PagesS115-S119

JournalChromatographia: An International Journal for Separation Science

Volume number57

ISSN0009-5893

eISSN1612-1112

DOI Linkhttps://doi.org/10.1007/BF02492092

Conference24th International Symposium on Chromatography

PublisherSpringer


Abstract
As a result of recent European Union regulations food colorants must be determined quantitatively, not just qualitatively as in the past. We demonstrate that a mixture of 14 synthetic food colorants (E 102, E 104, E 110, E 122, E 123, E 124, E 127 E 128, E 129, E 131, E 132, E 133, E 142, and E 151) can best be resolved by use of a Purospher RP18e column (125 mm x 4 mm i.c., 5 mum particle) and a gradient prepared from 100 mm acetate buffer (pH 70) and acetonitrile. Diode-array detection was used to monitor the colorants; limits of detection ranged from 0.02 ng (E 142) to 2.05 ng (E 132) at a signal-to-noise ratio of 3.



Citation Styles

Harvard Citation styleKirschbaum, J., Krause, C., Pfalzgraf, S. and Brückner, H. (2003) Development and evaluation of an HPLC-DAD method for determination of synthetic food colorants, Chromatographia: An International Journal for Separation Science, 57, pp. S115-S119. https://doi.org/10.1007/BF02492092

APA Citation styleKirschbaum, J., Krause, C., Pfalzgraf, S., & Brückner, H. (2003). Development and evaluation of an HPLC-DAD method for determination of synthetic food colorants. Chromatographia: An International Journal for Separation Science. 57, S115-S119. https://doi.org/10.1007/BF02492092



Keywords


column liquid chromatographyCONFECTIONERYdiode-array detectionDYESfood colorant analysisPONCEAU 4RSIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATIONSUNSET YELLOW FCFsynthetic food colorants

Last updated on 2025-02-04 at 00:02