Journal article

Publication year: 2003

Pages: 11-18

Journal: Biological Chemistry

Volume number: 384

Issue number: 1

ISSN: 1431-6730

DOI Link: https://doi.org/10.1515/BC.2003.002

Publisher: De Gruyter Brill

Abstract: 
Selenoprotein mRNAs are particular in several aspects. They contain a specific secondary structure in their 3'UTR, called Secis (selenocysteine inserting sequence), which is indispensable for selenocysteine incorporation, and they are degraded under seleniumlimiting conditions according to their ranking in the hierarchy of selenoproteins. In the familiy of seleniumdependent glutathione peroxidases (GPx) the ranking is GI-GPxgreater than or equal toPHGPx>cGPx=pGPx. This phenomenon was studied by mutually combining the coding regions of GI-GPx, PHGPx and cGPx with their 3'UTRs. HepG2 cells were stably transfected with the resulting constructs. Expression of glutathione peroxidases was estimated by activity measurement and Western blotting, the seleniumdependent mRNA stability by realtime PCR. Whereas 3'UTRs from stable PHGPx and GI-GPx could be exchanged without loss of stability, they were not able to stabilize cGPx mRNA. cGPx 3'UTR rendered GI-GPx and PHGPx mRNA unstable. Thus, cGPx mRNA contains seleniumresponsive instability elements in both the translated and the untranslated region, which cannot be compensated by one of the stable homologs. Stabilizing efficiency of an individual GPx 3U'TR did not correlate with the efficiency of selenocysteine incorporation. PHGPx 3'UTR was equally effective as cGPx 3'UTR in enhancing GPx activity in all constructs, while GI-GPx 3'UTR showed a markedly lower efficacy. We conclude that different mRNA sequences and/or RNAbinding proteins might regulate mRNA stability and translation of selenoprotein mRNA.

Harvard Citation style: Müller, C., Wingler, K. and Brigelius-Flohé, R. (2003) 3′UTRs of glutathione peroxidases differentially affect selenium-dependent mRNA stability and selenocysteine incorporation efficiency, Biological Chemistry, 384(1), pp. 11-18. https://doi.org/10.1515/BC.2003.002

APA Citation style: Müller, C., Wingler, K., & Brigelius-Flohé, R. (2003). 3′UTRs of glutathione peroxidases differentially affect selenium-dependent mRNA stability and selenocysteine incorporation efficiency. Biological Chemistry. 384(1), 11-18. https://doi.org/10.1515/BC.2003.002