Journal article

Publication year: 2002

Pages: 943-948

Journal: Journal of Investigative Dermatology

Volume number: 119

Issue number: 4

ISSN: 0022-202X

eISSN: 1523-1747

Open access status: Bronze

DOI Link: https://doi.org/10.1046/j.1523-1747.2002.00182.x

Publisher: Elsevier

Abstract: 
Choline is an essential component in acetylcholine biosynthesis, and is involved in cell signaling. It is unable to permeate the cell membrane and requires a transporter to enter the cell. Neurons that synthesize acetylcholine take up choline by a recently cloned high-affinity choline transporter (choline transporter 1) that is Na+-dependent and can be blocked by hemicholinium-3. The aim of this study was to determine the expression and to analyze the distribution of choline transporter 1 in human and rat skin. The mRNA for choline transporter 1 was detected in rat and human skin and in the human keratinocyte cell line HaCaT. A polyclonal anti-serum was developed against the N-terminal region of the human and rat protein. In rat and human skin, choline transporter 1 immunoreactivity was present in nerve fibers. In addition, keratinocytes, HaCaT cells and cells of the internal root sheath of the hair follicle contained choline transporter 1 immunoreactivity. The labeling patterns of nonconfluent vs confluent cultured cells and the distribution of choline transporter I along the epidermal layer suggest an association of choline transporter 1 with keratinocyte differentiation. In conclusion, this study shows the presence of the high-affinity choline transporter choline transporter 1 in nerve fibers and epithelial cells in the human and rat skin supporting the pivotal role of this transporter in both the neuronal and non-neuronal cholinergic system of the skin.

Harvard Citation style: Haberberger, R., Pfeil, U., Lips, K. and Kummer, W. (2002) Expression of the high-affinity choline transporter, CHT1, in the neuronal and non-neuronal cholinergic system of human and rat skin, Journal of Investigative Dermatology, 119(4), pp. 943-948. https://doi.org/10.1046/j.1523-1747.2002.00182.x

APA Citation style: Haberberger, R., Pfeil, U., Lips, K., & Kummer, W. (2002). Expression of the high-affinity choline transporter, CHT1, in the neuronal and non-neuronal cholinergic system of human and rat skin. Journal of Investigative Dermatology. 119(4), 943-948. https://doi.org/10.1046/j.1523-1747.2002.00182.x