Journal article

Publication year: 2002

Pages: 435-440

Journal: Histochemical Journal

Volume number: 34

Issue number: 8-9

ISSN: 0018-2214

DOI Link: https://doi.org/10.1023/A:1023691606047

Publisher: Springer Verlag (Germany)

Abstract: 
The neurotransmitter supply in the nerve endings of the mantle and the siphuncle, i.e. in organs that are responsible for the shell formation in the ectocholeate Nautilus pompilius, were investigated with electron microscopical, fluorescence-, immuno- and enzyme histochemical methods as well as with high pressure liquid chromatography (HPLC). Using antibodies against serotonin and the tetrapeptide FMRF-amide, positive reactions were demonstrated immunohistochemically within the terminal nerve fibres of the mantle and the vessels of the siphuncle. Enzyme histochemical proof of the presence of specific acetylcholinesterase yielded positive results in the muscle fibres of the mantle and siphuncle. Additionally, in the mantle, glyoxylic acid-induced fluorescence was shown within the nerve endings indicating catecholamines as neurotransmitters, whereas in the siphuncle such fluorescence did not appear. However, the HPLC-analyses showed that in the mantle and also in the siphuncle the content of dopamine is higher than that of noradrenaline whereas only traces of adrenaline occur in both organs suggesting dopamine as a putative neurotransmitter. Transmission electron microscopical examination of the nerve endings of both organs revealed that different types of vesicles were distinguished that could be considered as cholinergic, aminergic and peptidergic structures.

Harvard Citation style: Westermann, B., Beuerlein, K., Hempelmann, G. and Schipp, R. (2002) Localization of putative neurotransmitters in the mantle and siphuncle of the mollusc Nautilus pompilius L. (Cephalopoda), Histochemical Journal, 34(8-9), pp. 435-440. https://doi.org/10.1023/A:1023691606047

APA Citation style: Westermann, B., Beuerlein, K., Hempelmann, G., & Schipp, R. (2002). Localization of putative neurotransmitters in the mantle and siphuncle of the mollusc Nautilus pompilius L. (Cephalopoda). Histochemical Journal. 34(8-9), 435-440. https://doi.org/10.1023/A:1023691606047