Journalartikel

Jahr der Veröffentlichung: 2002

Seiten: 3905-3911

Zeitschrift: European Journal of Biochemistry

Bandnummer: 269

Heftnummer: 16

ISSN: 0014-2956

Open Access Status: Bronze

DOI Link: https://doi.org/10.1046/j.1432-1033.2002.03056.x

Verlag: Wiley: No OnlineOpen

Abstract: 
Palytoxin stimulated a cation-dependent short-circuit current (Isc) in rat distal and proximal colon in a concentration-dependent fashion when applied to the mucosal surface of the tissue. The distal colon exhibited a higher sensitivity to the toxin. The palytoxin-induced Isc was blocked by vanadate but was resistant to ouabain or scilliroside, suggesting the conversion of a vanadate-sensitive H+ /K+-ATPase into an electrogenic cation transporter. Cation substitution experiments with basolaterally depolarized tissues suggested an apparent permeability of the palytoxin-induced conductance of Na+ K+ > Li+. Immunohistochemical control experiments confirmed the absence of the Na+ /K+-ATPase in the apical membrane. Consequently, the pore-forming action of palytoxin is not restricted to Na+ /K+-ATPase but is also observed with the colonic H+ /K+-ATPase.

Harvard-Zitierstil: Scheiner-Bobis, G., Hübschle, T. and Diener, M. (2002) Action of palytoxin on apical H+/K+-ATPase in rat colon, European Journal of Biochemistry, 269(16), pp. 3905-3911. https://doi.org/10.1046/j.1432-1033.2002.03056.x

APA-Zitierstil: Scheiner-Bobis, G., Hübschle, T., & Diener, M. (2002). Action of palytoxin on apical H+/K+-ATPase in rat colon. European Journal of Biochemistry. 269(16), 3905-3911. https://doi.org/10.1046/j.1432-1033.2002.03056.x