Journalartikel

Jahr der Veröffentlichung: 2002

Seiten: 133-142

Zeitschrift: Neurogastroenterology and Motility

Bandnummer: 14

Heftnummer: 2

ISSN: 1350-1925

DOI Link: https://doi.org/10.1046/j.1365-2982.2002.00312.x

Verlag: Wiley: 12 months

Abstract: 
Myenteric neurones from 1-10-day-old rats were isolated from the small and large intestine by enzymatic digestion with collagenase. Single cells were collected and kept in culture for up to 1 week. After 1-5 days in culture, membrane potential and ionic currents were measured with the whole-cell patch-clamp technique. The intracellular Ca(2+) concentration was measured with the fura-2 method. The short-chain fatty acid butyrate (50 mmol L(-1) ) induced a reversible hyperpolarization of the myenteric neurones by about 10 mV. This hyperpolarization was concomitant with an inhibition of a TTX-sensitive Na(+) current. The hyperpolarization could be suppressed by intracellular application of Cs(+) , a nonselective K(+) channel blocker. Fura-2 experiments revealed that butyrate induced an increase of the intracellular Ca(2+) concentration. The butyrate response was suppressed by thapsigargin, indicating that butyrate stimulates the release of intracellular Ca(2+) . This release is responsible for the voltage response, because intracellular chelation of Ca(2+) inhibited the butyrate induced hyperpolarization. Consequently, butyrate acts on enteric neurones by releasing Ca(2+) from intracellular stores with the consequence of the activation of K(+) channels, followed by a hyperpolarization.

Harvard-Zitierstil: Haschke, G., Schäfer, H. and Diener, M. (2002) Effect of butyrate on membrane potential, ionic currents and intracellular Ca2+ concentration in cultured rat myenteric neurones, Neurogastroenterology and Motility, 14(2), pp. 133-142. https://doi.org/10.1046/j.1365-2982.2002.00312.x

APA-Zitierstil: Haschke, G., Schäfer, H., & Diener, M. (2002). Effect of butyrate on membrane potential, ionic currents and intracellular Ca2+ concentration in cultured rat myenteric neurones. Neurogastroenterology and Motility. 14(2), 133-142. https://doi.org/10.1046/j.1365-2982.2002.00312.x