Journal article

Publication year: 2001

Pages: 759-762

Journal: Steroids

Volume number: 66

Issue number: 10

ISSN: 0039-128X

DOI Link: https://doi.org/10.1016/S0039-128X(01)00109-X

Publisher: Elsevier

Abstract: 
A first assay based on stable isotope dilution/gas chromatography-mass spectrometry has been developed for plasma 17 alpha -hydroxypregnenolone, the leading marker of 3 beta -hydroxysteroid dehydrogenase deficiency. Equilibration of plasma with internal standard was followed by solid phase extraction. After clean up using Sephadex LH-20 mini columns, heptafluorobutyrates were prepared as derivatives. Quantification was achieved by selected ion monitoring of m/z 467.0 (analyte) and m/z 471.0 (internal standard). 0.030 pmol of 17a-hydroxypregnenolone gave a signal to noise ratio of 3.7. Calibration plot was linear. Spiking experiments showed good accuracy with relative errors < 3.7%. Intraassay precision CV was 8.3% and interassay precision CV was 5.6%. Requiring small amounts of plasma, the rapid, convenient work up and the application of bench top GC/MS instrumentation proved our method suitable for routine clinical use in adults and children. (C) 2001 Elsevier Science Inc. All rights reserved.

Harvard Citation style: Wudy, S., Hartmann, M., Solleder, C. and Homoki, J. (2001) Determination of 17α-hydroxypregnenolone in human plasma by routine isotope dilution mass spectrometry using benchtop gas chromatography-mass selective detection, Steroids, 66(10), pp. 759-762. https://doi.org/10.1016/S0039-128X(01)00109-X

APA Citation style: Wudy, S., Hartmann, M., Solleder, C., & Homoki, J. (2001). Determination of 17α-hydroxypregnenolone in human plasma by routine isotope dilution mass spectrometry using benchtop gas chromatography-mass selective detection. Steroids. 66(10), 759-762. https://doi.org/10.1016/S0039-128X(01)00109-X